Biochemical and Biophysical Research Communications
Modulation of hypothalamic hypocretin/orexin mRNA expression by glucocorticoids
Section snippets
Materials and methods
Sham and bilaterally adrenalectomized (ADX) male Sprague–Dawley rats (body weight 250 g, IFFA CREDO, France) were individually housed in plastic cages with a stainless steel cover in a temperature-controlled room on a 12:12 h light/dark cycle (light on at 06h00). They were allowed ad libitum access to tap water supplemented with 9 g/L NaCl and standard rat laboratory chow diet. Three experimental groups were made (n=8 per group), a sham-operated control group treated with vehicle, an
Results
Fig. 1 shows the central distribution of prepro-Hcrt/OX mRNA using in situ hybridization in rat brain coronal section. In control rat, a strong hybridization signal was observed in the lateral hypothalamus with extension into the perifornical nucleus and the posterior hypothalamic area. In ADX rats, the signal persisted in the lateral hypothalamus, but less intense, with fewer hybridization signals in the perifornical nucleus and the posterior hypothalamic area. Almost all hybridization signals
Discussion
In the present report, we investigated the regulation of Hcrt/OX pathway by glucocorticoids by measuring Hcrt/OX brain expression in the presence or absence of corticosterone. Firstly, we confirmed that prepro-Hcrt/OX mRNA expression is restricted to the lateral hypothalamus area with extension to the perifornical nucleus and the posterior hypothalamic area [1], [2]. This pattern of expression is consistent with a role of Hcrt/OX peptides in the control of food intake [3], [4], [5], [6] and
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