TY - JOUR T1 - Antibodies to Pharmacologically Active Molecules: Specificities and Some Applications of Antiprostaglandins JF - Pharmacological Reviews JO - Pharmacol Rev SP - 293 LP - 307 VL - 25 IS - 2 AU - Lawrence Levine Y1 - 1973/06/01 UR - http://pharmrev.aspetjournals.org/content/25/2/293.abstract N2 - Because of the low concentrations of these prostaglandin compounds in tissues and fluids and because of the large number of closely related structures, analytical procedures for their quantitative and qualitative estimation are not adequate or are restricted to only a few laboratories. Specific antibodies can now quantitate the following compounds: PGF2α, PGF1α, 15-keto-PGF2α, 13, 14-dihydro-15-keto-PGF2α, and 13,14-dihydro-PGF2α. For some analyses, the samples can be assayed and identified with only one antiserum. For other analyses, a combination of antisera must be used for positive identification. Antisera directed toward PGB, which indirectly measure POE or PGA or both, i.e., POE or PGA can be converted to PGB by treatment with NaOH, are also available; it is hoped that soon antisera that can measure the PGE metabolites will be produced. Knowledge of the levels of prostaglandins, prostaglandin metabolites, and activities of the prostaglandin synthetic and metabolic enzymes in tissues and biological fluids would help to establish a physiological role(s) for the prostaglandin system. 1971, by The Williams & Wilkins Co. ER -