RT Journal Article
SR Electronic
T1 Disposition of drugs in man by radioimmunoassay.
JF Pharmacological Reviews
JO Pharmacol Rev
FD American Society for Pharmacology and Experimental Therapeutics
SP 73
OP 76
VO 34
IS 1
A1 S Spector
YR 1982
UL http://pharmrev.aspetjournals.org/content/34/1/73.abstract
AB The disposition of morphine and clonidine was investigated by means of radioimmunoassay. After the administration of a single i.v. dose of morphine (10 mg/70 kg) to 10 men who had not received other drugs for two weeks preceding the study, a multiphasic decline in serum concentrations of morphine occurred. Detectable blood concentrations of morphine, or a metabolite, or of both, persisted for up to 96 hours after a single i.v. dose. The radioimmunoassay for clonidine is capable of detecting as little as 10 pg of clonidine. The antibody fails to bind 2,6-dichlorophenylguanidine, a known metabolite of clonidine, while the other metabolite, 4-hydroxyclonidine, was as potent as clonidine in displacing labeled clonidine from the antibody. The disposition of clonidine ion plasma and tissues could be monitored by radioimmunoassay.