Antagonist | Antagonist KB value (μM) | Binding studies (Ki, μM) | |||
---|---|---|---|---|---|
Slices8-a | Dissociated cells8-b | Homogenates8-c,8-d,8-e | Krebs buffer | Tris buffer | |
Cimetidine | 0.6 | 0.5 | 0.9 | ||
Metiamide | 0.8 | n.d. | 1.0 | ||
Tiotidine | n.d. | 0.03 | 0.03 | 0.02 | 0.007 |
Cyproheptadine | 5.7 | n.d. | 0.04 | ||
Mianserin | 10.0 | 2.8 | 0.07 | 1.01 | 0.20 |
Imipramine | >10 | 3.3 | 0.2 | ||
Amitriptyline | 3.5 | 1.9 | 0.05 | 0.09 | 0.09 |
Chlorpromazine | 5.9 | 3.0 | 0.04 | ||
Haloperidol | >10 | 29 | 0.08 | 1.61 | 0.42 |
Measurements were made of H2-mediated adenylyl cyclase activity in homogenates of guinea pig hippocampus, impromidine-stimulated cyclic AMP accumulation in guinea pig hippocampal slices, and of H2-mediated cyclic AMP accumulation in dissociated hippocampal tissue. n.d., not determined.
↵8-a Tuong et al. (1980)
↵8-b Kamba et al. (1983)
↵8-c Green et al. (1977)
↵8-d Kanof and Greengard (1978)
↵8-e Kanof and Greengard (1979a,b)
8-f Traiffort et al. (1991)