Class of molecules | Molecule | Molecular weight (kDa) | Cloning and sequencing1-a |
---|---|---|---|
Substrates | HMW-kininogen | 88–120 | A differentially spliced single gene product codes for both (Margoliuset al., 1995) |
LMW-kininogen | 50–68 | ||
Kininogenases | Plasma prekallikrein | 82–83 (Chung et al., 1986) | 619 a.a. mature protein; 19 a.a. signal peptide; (Chung et al., 1986) |
Tissue kallikrein hK1 | 40–45 (Chen et al., 1995a) | 238 a.a. mature protein; 24 a.a. profragment (Fukushima et al., 1985) | |
Tissue kallikrein hK2 | 40–45 (Schedlich et al., 1987) | 261 a.a. preproprotein; 237 a.a. mature protein; (Schedlich et al., 1987) | |
Kallikrein inhibitors | C1q-inhibitor | 21–750 range; 30 (core protein) (Ghebrehiwet and Galankis, 1993) | Not available |
Kallistatin, a serpin | 58 (Zhou et al., 1992) | 401 a.a. mature protein; 26 a.a. signal peptide; (Chai et al., 1993) | |
Kininases | Carboxypeptidase N | 50 (catalytic subunit); 83 (carrier subunit) (Tan et al., 1990) | 438 a.a. mature protein + 20 a.a. signal peptide (catalytic subunit—Gebhardet al., 1989) 536 a.a. carrier subunit (Tan et al., 1990) |
Carboxypeptidase M | 62 (Skidgel et al., 1989) | 439 a.a. protein (Tan et al., 1989) | |
Kininase II; angiotensin I converting enzyme | 170 (Takeuchiet al., 1989) | 1306 a.a. total with 29 a.a. signal peptide (Soubrier et al., 1988) | |
Neutral endopeptidase | 100 (Shipp et al., 1988) | 749 a.a. protein (Shipp et al., 1989) | |
Aminopeptidase M | 150, 110 and 56/52 (Terashima and Bunnett, 1995) | 966 a.a. (in the rat—Malfroy et al., 1989) | |
Aminopeptidase P | 91 (Hyde et al., 1996) | 673 a.a. (in the pig—Hydeet al., 1996) | |
G protein-coupled receptors | Receptor B1 | Predicted (without carbohydrates): 40 | 353 a.a. G protein-coupled receptor (Menke et al., 1994) |
Receptor B2 | Predicted (without carbohydrates): 41; found 69 | 364 a.a. G protein-coupled receptor (Hess et al., 1992) |
↵1-a The human sequence is reported, unless otherwise indicated.