Table 3

PAR-1 regulated kinases

Kinase Pathway	Intermediate/G-protein	Functional Response	Cell Type	References
p44 MAP kinase (ERK)	p21^RAS/Gi/Go, tyrosine kinase	ND	Rat-1 fibroblasts	Van Corven et al., 1993
ND	p21^RAS	AP-1/DNA synthesis	Astrocytoma cells	La Morte et al., 1993
p42/44 MAP kinase	SHC/GRB-2	ND	CCL39 fibroblasts	Chen et al., 1996
p42/44 MAP kinase	RAF-1, tyrosine kinase	ND	NIH3T3 fibroblasts	Apostolidis and Weiss, 1997
ERK-5	G₁₂/G₁₃/G_q	ND	COS-7	Fukuhara et al., 2000
JNK/p38 MAP kinase	PKC	ND	HepG2	Mitsui et al., 1998
pp60^src	G_i/G_o, G_q?	ND	CCL339	Chen et al., 1994
pp72^Syk	ND	ND	Platelets	Satoh et al., 1996
rasGAP	Fyn, Lyn Yes	ND	Platelets	Cichowski et al., 1992
SH-PTP-1, PTP1D	ND	Cytoskeleton, growth	Platelets, CCL39 cells	Rivard et al., 1995
p70^56K	P-13 kinase, PKC, G_i/G_o	DNA synthesis	Pulmonary artery fibroblasts, HTSM, BTSM	Belham et al., 1997; Krymskaya, et al., 1999; Walker et al., 1998
Protein kinase B	PI-3 kinase	DNA synthesis	BTSMC	Walker et al., 1998
PI-3 kinase	ND	Blebbling	CHRF-288	Vemuri et al., 1996
PI-3	Rho, βγ-subunits	ND	Platelets	Zhang et al., 1995
JAK-2/STAT-1	ND	Inhibition of growth	Tumor cells	Huang et al., 2000
PYK-2	Src, PKC	Aggregation	Platelets	Ohmori et al., 1999
ND	GEF/Rho/G₁₂/G₁₃	Cell rounding	1321N1 astrocytoma cells	Majumdar et al., 1999
Rho kinase	Rho	DNA synthesis/migration	RASMC	Seasholtz et al., 1999
pp125^FAK	ND	ND	Glomerula mesangial cells	Choudhury et al., 1996

Kinase pathways activated following PAR-1 stimulation. Intermediates in all cases have not been identified and are partly based on other G-protein-coupled receptors. JAK-2, Janus-activated kinase-2; Src, cellular homology of the Src oncogene product; STAT-1, signal transducers and activator of transcrition-1; GAP, GTPase activating protein; SYK, spleen tyrosine kinase; PYK2, proline-rich tyrosine kinase 2; SH-PTP-1, Src homology domain containing protein tyrosine phosphatase-1; GEF, Rho G-protein nucleotide exchange factor; ND, not determined.