Airways | Cattle | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 mM |
Ethier and Madison, 2002
|
Airways | Cattle | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 mM |
Bazan-Perkins et al., 2001
|
Airways | Human | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 mM |
Ethier and Madison, 2002
|
Airways | Human | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 30 mM |
Iizuka et al., 1998
|
Anococcygeus | Mouse | Isolated smooth muscle cells; whole-cell patch-clamping; ICl(Ca), IDOC | 10 mM |
Wayman et al., 1998
|
Anococcygeus | Mouse | Isolated smooth muscle cells; whole-cell patch-clamping; ICl(Ca), IDOC | 10 mM |
Wayman et al., 1997
|
Aorta | Guinea pig | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 20 mM |
Nixon et al., 1994
|
Aorta | Pig | Microsomes, F3 microsomal membranes fraction; inorganic phosphate-release assay; Ca2+-ATPase activity | 20 mM |
Luo et al., 2000
|
Aorta | Rabbit | Isolated intact or saponin-permeabilized smooth muscle cells; mag-fura-2 cellular loading, fluorometry; [Ca2+]SR, [Ca2+]mito | 10–20 mM |
Gurney et al., 2000
|
Aorta | Rabbit | Isolated endothelium-denuded strips; fura-2-cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 mM |
Ahn and Karaki, 1988
|
Aorta | Rabbit | Isolated rings; isometric dynamometry; contractile activity | 20 mM |
Luo et al., 2000
|
Aorta | Rat | Microsomes; 45Ca2+ loading; Ca2+ fluxes | 20 mM |
Yusufi et al., 2002
|
Aorta | Rat | Isolated cultured saponin-permeabilized smooth muscle cells; 45Ca2+ cellular loading; Ca2+ fluxes | 30 mM |
Yamamoto et al., 1991
|
Aorta | Rat | Isolated smooth muscle cells; fura-2 cellular loading, epifluorescence microscopy; [Ca2+]cyt imaging | 5–40 mM |
Vallot et al., 2001
|
Aorta | Rat | Isolated endothelium-denuded strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 20 mM |
Sato et al., 1988
|
Basilar artery | Rabbit | Isolated endothelium-denuded strips; isometric dynamometry; contractile activity | 10 mM |
Szado et al., 2001
|
Carotid artery | Rat | Isolated endothelium-denuded strips; isometric dynamometry; contractile activity | 20 mM |
Nomura and Asano, 2000
|
Cerebral artery | Mouse | Isolated smooth muscle cells and segments, phospholamban-knockout mice; whole-cell amphotericin B-perforated patch-clamping, fura-2 or fluo-3 cellular loading, confocal fluorescence microscopy; IBK, [Ca2+]cyt | 10 mM |
Wellman et al., 2001
|
Colon | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fluo-3 cellular loading, fluorometry or wide-field fluorescence digital imaging; ICa, [Ca2+]cyt, [Ca2+]cyt imaging | 10 mM |
Bradley et al., 2003
|
Colon | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fluo-3 or fura-2 cellular loading, fluorometry; ICa, [Ca2+]cyt | 10 mM |
Bradley et al., 2002
|
Colon | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fluo-3 cellular loading, epifluorescence microscopy; membrane currents, [Ca2+]cyt | 10 mM |
Flynn et al., 2001
|
Colon | Mouse | Isolated smooth muscle cells; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 1 mM |
Bayguinov et al., 2001
|
Colon | Mouse | Isolated smooth muscle cells; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 1 mM |
Bayguinov et al., 2000
|
Colon | Guinea pig | Isolated smooth muscle cells; fluo-3 cellular loading, wide-field fluorescence digital imaging; [Ca2+]cyt imaging | 10 mM | McCarron et al., 2004 |
Coronary artery | Cattle | Microsomal membranes stripped or not of FKBP-12.6, reconstituted into planar lipid bilayers; bilayer clamp amplification; single Ca2+ channel currents | 1–5 mM |
Li et al., 2001
|
Coronary artery | Dog | Isolated fibers; isometric dynamometry; contractile activity | 25 mM |
Imai et al., 1984
|
Coronary artery | Pig | Isolated intact or saponin-permeabilized smooth muscle cells; quin-2 cellular loading, 45Ca2+ cellular loading (intact or permeabilized); cellular Ca2+ fluxes, Ca2+ fluxes from intracellular stores, [Ca2+]cyt | 20 mM |
Ueno et al., 1987
|
Coronary artery | Pig | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 5 mM |
Heaps et al., 2001
|
Cremaster muscle arterioles | Rat | Isolated, cannulated and pressurized endothelium-denuded segments; fura-2 cellular loading, fluorometry, diameter monitoring by video microscopy; [Ca2+]cyt, contractile activity | 20 mM |
Potocnik and Hill, 2001
|
Esophagus | Human | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 5 mM |
Sims et al., 1997
|
Femoral artery | Rabbit | Isolated endothelium-denuded rings; isometric dynamometry; contractile activity | 20 mM |
Jezior et al., 2001
|
Femoral artery | Rat | Isolated endothelium-denuded strips; isometric dynamometry; contractile activity | 20 mM |
Asano and Nomura, 2001
|
Femoral artery | Rat | Isolated endothelium-denuded strips; isometric dynamometry; contractile activity | 20 mM |
Nomura and Asano, 2000
|
Gall bladder | Guinea pig | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping; IBK | 250 μM and 1 mM |
Pozo et al., 2002
|
Ileum | Guinea pig | Isolated intact smooth muscle cells and β-escin-permeabilized strips; whole-cell and outside-out patch-clamping, indo-1 cellular loading, fluorometry, isometric dynamometry; IBK, STOCs, [Ca2+]cyt, contractile activity | 3–30 mM 30 and 300 μM (MBED) |
Imaizumi et al., 1996
|
Ileum | Guinea pig | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 30 mM |
Iizuka et al., 1998
|
Ileum (circular layer) | Guinea pig | Isolated β-escin-permeabilized strips; isometric dynamometry; contractile activity | 20 mM |
Fukami et al., 1993
|
Mesenteric artery | Guinea pig | Isolated smooth muscle cells; microelectrode, alumina adsorption; membrane potential, excitatory junction potential | 10 μM–5 mM |
Fujii et al., 1985
|
Mesenteric artery | Rabbit | Isolated saponin-permeabilized strips; isometric dynamometry; contractile activity | 3 and 25 mM |
Kanmura et al., 1989
|
Mesenteric artery | Rat | Isolated cultured smooth muscle cells; furaptra cellular loading, fluorometry; [Ca2+]SR | 10 mM |
Golovina and Blaustein, 1997
|
Mesenteric artery | Rat | Isolated endothelium-denuded strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 2 and 20 mM |
Akata et al., 2001
|
Mesenteric artery | Rat | Isolated endothelium-denuded strips; isometric dynamometry; contractile activity | 20 mM |
Nomura and Asano, 2000
|
Mesenteric resistance arteries | Rat | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 mM |
Zang et al., 2001
|
Mesenteric resistance arteries | Rat | Isolated endothelium-denuded strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 mM |
Lagaud et al., 1999
|
Mesenteric resistance arteries | Rat | Isolated 3rd-order branches; isometric dynamometry; contractile activity | 10 mM |
Rubio et al., 2002
|
Mesenteric resistance arteries | Rat | Isolated, cannulated and pressurized endothelium-intact or -denuded segments isolated from 3rd- and 4th-order branches; fluo-4 cellular loading, confocal fluorescence microscopy, diameter monitoring by video microscopy; [Ca2+]cyt imaging, contractile activity | 20 μM | Lamont and Wier, 2004 [in press] |
Mesenteric resistance arteries | Rat | Isolated, cannulated and pressurized vessels isolated from 5th-order branches; diameter monitoring by video microscopy; contractile activity | 10 μM |
Giulumian et al., 2000
|
Portal vein | Guinea pig | Isolated β-escin-permeabilized strips; isometric dynamometry; contractile activity | 3–30 mM 30 and 300 μM (MBED) |
Imaizumi et al., 1996
|
Portal vein | Guinea pig | Isolated strips; isometric dynamometry; contractile activity | 1–100 mM |
Yagi et al., 1985
|
Portal vein | Guinea pig | Saponin-permeabilized smooth muscle cells and strips; microelectrode, isometric dynamometry; membrane potential, contractile activity | 1–10 mM |
Nanjo, 1984
|
Portal vein | Rabbit | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 mM |
Povstyan et al., 2003
|
Pulmonary artery | Cattle | Microsomes; extramicrosomal fluo-3 and fluorometry or 45Ca2+ loading; Ca2+ fluxes | 20 mM |
Stout et al., 2002
|
Pulmonary artery | Rabbit | Isolated smooth muscle cells and endothelium-denuded strips; fura-2 cellular loading, fluorometry; [Ca2+]cyt, contractile activity | 10 mM |
Dipp et al., 2001
|
Pulmonary artery | Rabbit | Isolated smooth muscle cells and strips; double sucrose gap, microelectrode, isometric dynamometry; membrane potential, ionic current, contractile activity | 0.1–10 mM |
Ito et al., 1977
|
Pulmonary resistance arteries | Dog | Smooth muscle cells isolated from 3rd- and 4th-order branches; fura-2 and fluo-3 or fluo-4 cellular loading, fluorometry, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 mM |
Janiak et al., 2001
|
Pulmonary resistance arteries | Rabbit | Isolated saponin-permeabilized smooth muscle cells; whole-cell patch-clamping; membrane potential | 10 mM |
Wilson et al., 2001
|
Pulmonary resistance arteries | Rabbit | Smooth muscle cells isolated from 3rd- and 4th-order branches; whole-cell patch-clamping, fura-2 cellular loading, fluorometry; membrane current, [Ca2+]cyt | 5 mM |
Smani et al., 2001
|
Pulmonary resistance arteries | Rat | Isolated endothelium-denuded rings; isometric dynamometry; contractile activity | 10 mM |
Dipp and Evans, 2001
|
Renal pelvis | Guinea pig | Isolated urothelium-denuded strips; microelectrode, isometric dynamometry; membrane potential, contractile activity | 1 mM |
Lang et al., 2002
|
Renal resistance arteries | Dog | Smooth muscle cells isolated from 3rd- and 4th-order branches; fura-2 and fluo-3 or fluo-4 cellular loading, fluorometry, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 mM |
Janiak et al., 2001
|
Small intestine | Mouse | Isolated segments; microelectrode; slow wave frequency | 5 mM |
Malysz et al., 2001
|
Stomach | Mouse | Isolated smooth muscle cells and strips; whole-cell patch-clamping, fura-2 cellular loading, fluorometry, isometric dynamometry; ICch, STOCs, [Ca2+]cyt, contractile activity | 5–10 mM |
Tokutomi et al., 2001
|
Stomach (antrum) | Cattle | Microsomes; extramicrosomal fluo-3 and fluorometry or 45Ca2+ loading; Ca2+ fluxes | 20 mM |
Stout et al., 2002
|
Stomach (antrum) | Rat | Isolated smooth muscle cells; Oregon Green BAPTA 5 cellular loading, fluorometry; [Ca2+]SR | 10 mM |
White and McGeown, 2002
|
Stomach (antrum, circular layer) | Guinea pig | Isolated smooth muscle bundles; microelectrode, fura-2 cellular loading, fluorometry; membrane potential, [Ca2+]cyt | 1 mM |
Fukuta et al., 2002
|
Stomach (antrum, circular layer) | Guinea pig | Isolated smooth muscle strips; isometric dynamometry; contractile activity | 1–10 mM |
Itoh et al., 1982b
|
Stomach (pylorus) | Guinea pig | Isolated smooth muscle strips; microelectrode; slow wave frequency | 0.3–1 mM |
Van Helden et al., 2000
|
Taenia caeci | Guinea pig | Isolated strips; isometric dynamometry; contractile activity | 1–100 mM |
Yagi et al., 1985
|
Taenia coli | Guinea pig | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping; STOCs | 1 mM |
Kong et al., 2000
|
Tail artery | Rat | Isolated freshly or cultured cells and endothelium-denuded rings; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 5 mM |
Dreja et al., 2001
|
Trachea | Cat | Isolated smooth muscle cells; whole-cell nystatin perforated patch-clamping; ICch | 10 mM |
Waniishi et al., 1998
|
Trachea | Cat | Isolated mucosa- and adventitia-denuded strips; double sucrose-gap, isometric dynamometry; membrane potential, contractile activity | 10 mM |
Ito and Itoh, 1984
|
Trachea | Cattle | Isolated smooth muscle cells and strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 mM |
Tao et al., 2000
|
Trachea | Dog | Isolated cultured smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 5 mM |
Mitchell et al., 2000
|
Trachea | Rabbit | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 30 mM |
Iizuka et al., 1998
|
Ureter | Rat | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10–20 mM |
Burdyga et al., 2003
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fura-2 (for [Ca2+]cyt) or mag-fura-2 (for [Ca2+]SR) cellular loading, fluorometry; STOCs, [Ca2+]cyt, [Ca2+]SR | 20 mM |
Gomez-Viquez et al., 2003
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fura-2 cellular loading, fluorometry; membrane potential, ionic current, [Ca2+]cyt | 20 mM |
Wu et al., 2002
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 20 mM |
Rueda et al., 2002a
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, indo-1 cellular loading, confocal fluorescence microscopy; STOCs, [Ca2+]cyt imaging | 1–10 mM |
Ohi et al., 2001a
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, indo-1 cellular loading, fluorometry; IK, [Ca2+]cyt | 20 mM |
Weidelt and Isenberg, 2000
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell and outside-out patch-clamping, indo-1 cellular loading, fluorometry; IBK, STOCs, [Ca2+]cyt | 3–30 mM 30 and 300 μM (MBED) |
Imaizumi et al., 1996
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; indo-1 cellular loading, fluorometry; [Ca2+]cyt | 10 mM |
Ganitkevich and Isenberg, 1992
|
Urinary bladder | Rabbit | Isolated urothelium-denuded strips; isometric dynamometry; contractile activity | 20 mM |
Jezior et al., 2001
|
Uterus | Human | Isolated myometrial strips; indo-1 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 mM |
Kupittayanant et al., 2002
|
Vas deferens | Guinea pig | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 mM |
White and McGeown, 2003
|
Vas deferens | Guinea pig | Isolated smooth muscle cells; inside-out patch-clamping; IBK | 5 mM |
Ohi et al., 2001b
|
Vas deferens | Guinea pig | Isolated β-escin-permeabilized strips; isometric dynamometry; contractile activity | 20 mM |
Nixon et al., 1994
|
Vena cava (inferior) | Rabbit | Isolated smooth muscle cells and endothelium-denuded rings; fura-2 and fluo-3 cellular loading, fluorometry (fura-2), confocal fluorescence microscopy (fluo-3), isometric dynamometry; [Ca2+]cyt imaging, contractile activity | 0.5–50 mM |
Ruehlmann et al., 2000
|
Ventricles | Rabbit | Isolated smooth muscle cells; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 mM |
Haddock et al., 1999
|