Airways | Mouse | Lung slices; Oregon Green cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 200 μM |
Bergner and Sanderson, 2002
|
Airways | Pig | Isolated intact or β-escin-permeabilized cells; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt | 20 μM |
Pabelick et al., 2001a
|
Anococcygeus | Mouse | Isolated smooth muscle cells; whole-cell patch-clamping; IDOC, ICl(Ca) | 3 and 30 μM |
Wayman et al., 1997
|
Anococcygeus | Mouse | Isolated smooth muscle cells and whole tissue; whole-cell patch-clamping, isometric dynamometry; IDOC, ICl(Ca), contractile activity | 3–30 μM |
Wayman et al., 1998
|
Aorta | Rabbit | Isolated intact or saponin-permeabilized smooth muscle cells; mag-fura-2 cellular loading, fluorometry; [Ca2+]SR, [Ca2+]mito | 20 μM |
Gurney et al., 2000
|
Aorta | Rat | Microsomes; 45Ca2+ loading; Ca2+ fluxes | 30 nM ([3H]-ryanodine) |
Yusufi et al., 2002
|
Aorta | Rat | Isolated endothelium-denuded rings; isometric dynamometry; contractile activity | 30 μM |
Low et al., 1993
|
Basilar artery | Mouse | Isolated smooth muscle cells; whole-cell nystatin perforated patch-clamping; IBK | 10 μM |
Lohn et al., 2001
|
Basilar artery | Rabbit | Isolated smooth muscle cells and endothelium-denuded strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 μM |
Szado et al., 2001
|
Cerebellar artery | Rabbit | Isolated endothelium-intact or -denuded strips; isometric dynamometry; contractile activity | 10 μM | Jewell et al., 2004 |
Cerebral artery | Human | Isolated smooth muscle cells; fluo-3 cellular loading, confocal fluorescence microscopy, isometric dynamometry; [Ca2+]cyt imaging, contractile activity | 10 μM | Wellman et al., 2002 |
Cerebral artery | Mouse | Isolated segments, phospholamban-knockout mice; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 μM |
Wellman et al., 2001
|
Cerebral artery | Rabbit | Isolated endothelium-intact or -denuded strips; isometric dynamometry; contractile activity | 10 μM | Jewell et al., 2004 |
Cerebral artery | Rat | Isolated, cannulated and pressurized segments; fluo-3 cellular loading, confocal fluorescence microscopy, diameter monitoring by video microscopy; [Ca2+]cyt imaging, contractile activity | 10 μM |
Jaggar, 2001
|
Colon | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fluo-3 cellular loading, epifluorescence microscopy; membrane currents, [Ca2+]cyt | 50 μM |
Flynn et al., 2001
|
Colon | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fluo-3 or fura-2 cellular loading, fluorometry; ICa, [Ca2+]cyt | 50 μM |
Bradley et al., 2002
|
Colon | Mouse | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping, fluo-3 cellular loading, confocal fluorescence microscopy; STOCs, [Ca2+]cyt imaging | 10 μM |
Bayguinov et al., 2000
|
Colon (circular layer) | Dog | Isolated non-inflamed and inflamed saponin-permeabilized smooth muscle cells; fura-2 cellular loading or 45Ca2+ cellular loading, cell length monitoring by phase-contrast microscope; Ca2+ fluxes, [Ca2+]cyt, contractile activity | 10 μM |
Shi and Sarna, 2000
|
Coronary artery | Cattle | Microsomal membranes, stripped or not of FKBP-12.6, reconstituted into planar lipid bilayer; patch-clamping, bilayer clamp amplification; open channel probability (path clamping), single Ca2+ release channel currents (bilayer clamp amplification) | 0.1–50 μM |
Li et al., 2001
|
Coronary artery | Cattle | Isolated α-toxin-permeabilized smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 μM |
Yu et al., 2000
|
Coronary artery | Cattle | Isolated cultured smooth muscle cells and microsomes, FKBP12.6-stripped; patch-clamping, bilayer clamp amplification; open channel probability (path clamping), single Ca2+ release channel currents (bilayer clamp amplification) | 0.1 μM |
Tang et al., 2002
|
Coronary artery | Cow | Isolated smooth muscle cells, and cannulated and pressurized endothelium-denuded rings; fura-2 cellular loading, fluorometry, diameter monitoring by video microscopy; [Ca2+]cyt, contractile activity | 30 μM |
Ge et al., 2003
|
Coronary artery | Human | Isolated smooth muscle cells; whole-cell nystatin perforated patch-clamping, fluo-3 cellular loading, confocal fluorescence microscopy; IK(Ca), [Ca2+]cyt imaging | 50 μM |
Furstenau et al., 2000
|
Coronary artery | Rabbit | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 5 μM |
Kang et al., 2002
|
Esophagus | Human | Isolated smooth muscle cells and strips; fura-2 cellular loading, fluorometry, cell length monitoring by phase-contrast microscopy; [Ca2+]cyt, contractile activity | 1 μM |
Sims et al., 1997
|
Femoral artery | Rat | Isolated endothelium-denuded strips; fura-PE3 cellular loading, isometric dynamometry; [Ca2+]cyt, contractile activity | 0.1–10 μM |
Asano and Nomura, 2001
|
Gall bladder | Guinea pig | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping; IBK | 10 μM |
Pozo et al., 2002
|
Ileum | Guinea pig | Isolated smooth muscle cells; whole-cell and outside-out patch-clamping; IBK | 10 μM |
Imaizumi et al., 1996
|
Irideal arterioles | Rat | Isolated, cannulated and pressurized segments; diameter monitoring by video microscopy; contractile activity | 10 μM |
Haddock et al., 2002
|
Mesenteric arterioles | Rat | Isolated, cannulated and pressurized segments; fura-2 cellular loading, fluorometry, diameter monitoring by video microscopy; [Ca2+]cyt, contractile activity | 10 μM |
Watanabe et al., 1993
|
Mesenteric artery | Dog | Isolated endothelium-denuded rings; isometric dynamometry; contractile activity | 30–100 μM |
Low et al., 1992
|
Mesenteric resistance arteries | Rat | Isolated 3rd-order branches; isometric dynamometry; contractile activity | 20 μM |
Rubio et al., 2002
|
Mesenteric resistance arteries | Rat | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 μM |
Zang et al., 2001
|
Mesenteric resistance arteries | Rat | Isolated endothelium-denuded strips; isometric dynamometry; contractile activity | 10 μM |
Lagaud et al., 1999
|
Mesenteric resistance arteries | Rat | Isolated, cannulated and pressurized endothelium-intact or -denuded segments isolated from 3rd- and 4th-order branches; fluo-4 cellular loading, confocal fluorescence microscopy, isotonic dynamometry; [Ca2+]cyt imaging, contractile activity | 40 μM | Lamont and Wier, 2004 [in press] |
Mesenteric resistance arteries | Rat | Isolated, cannulated and pressurized vessels isolated from 5th-order branches; diameter monitoring by video microscopy; contractile activity | 10 μM |
Giulumian et al., 2000
|
Portal vein | Rabbit | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 1 μM |
Gordienko et al., 2001
|
Pulmonary artery | Cattle | Microsomes; extramicrosomal fluo-3 and fluorometry or 45Ca2+ loading; Ca2+ fluxes | 10 nM–750 μM |
Stout et al., 2002
|
Pulmonary artery | Rabbit | Isolated smooth muscle cells and endothelium-denuded strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 μM |
Dipp et al., 2001
|
Pulmonary artery | Rat | Physiological salt solution-perfused lungs; perfusion pressure; vascular bed resistance | 10–300 μM |
Morio and McMurtry, 2002
|
Pulmonary resistance arteries | Dog | Smooth muscle cells isolated from 3rd- and 4th-order branches; fura-2 and fluo-3 or fluo-4 cellular loading, fluorometry (fura-2), confocal fluorescence microscopy (fluo-3 and fluo-4); [Ca2+]cyt imaging | 10–300 μM |
Janiak et al., 2001
|
Pulmonary resistance arteries | Rabbit | Isolated saponin-permeabilized smooth muscle cells; whole-cell patch-clamping; membrane potential | 10 μM |
Wilson et al., 2001
|
Pulmonary resistance arteries | Rat | Isolated endothelium-denuded rings; isometric dynamometry; contractile activity | 10 μM |
Dipp and Evans, 2001
|
Renal pelvis | Guinea pig | Isolated urothelium-denuded strips; microelectrode, isometric dynamometry; membrane potential, contractile activity | 30 μM |
Lang et al., 2002
|
Renal resistance arteries | Dog | Smooth muscle cells isolated from 3rd- and 4th-order branches; fura-2 and fluo-3 or fluo-4 cellular loading, fluorometry, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10–300 μM |
Janiak et al., 2001
|
Skeletal muscle arterioles | Rat | Isolated, cannulated and pressurized segments; fura-2 cellular loading, fluorometry, diameter monitoring by video microscopy; [Ca2+]cyt, contractile activity | 10 μM |
Watanabe et al., 1993
|
Small intestine | Mouse | Isolated segments; microelectrode; slow wave frequency | 50 μM |
Malysz et al., 2001
|
Stomach | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping; IK(Ca) | 10 μM |
Duridanova et al., 1996
|
Stomach | Mouse | Isolated smooth muscle cells; whole-cell patch-clamping, fura-2 cellular loading, fluorometry; ICch, STOCS, [Ca2+]cyt | 1 μM |
Tokutomi et al., 2001
|
Stomach | Rat | Isolated smooth muscle cells; microelectrode, fura-2 cellular loading, fluorometry; ICa, [Ca2+]cyt | 10 μM |
White and McGeown, 2000
|
Stomach (antrum) | Cattle | Microsomes; extramicrosomal fluo-3 and fluorometry or 45Ca2+ loading; Ca2+ fluxes | 10 nM–750 μM |
Stout et al., 2002
|
Stomach (antrum, circular layer) | Guinea pig | Isolated smooth muscle bundles; microelectrode, fura-2 cellular loading, fluorometry; membrane potential, [Ca2+]cyt | 10 μM |
Fukuta et al., 2002
|
Taenia coli | Guinea pig | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping; STOCs | 10 μM |
Kong et al., 2000
|
Tail artery | Rat | Isolated freshly or cultured endothelium-denuded rings; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 10 μM |
Dreja et al., 2001
|
Testicular peritubules | Rat | Isolated saponin-permeabilized smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 5 μM |
Barone et al., 2002
|
Trachea | Cat | Isolated smooth muscle cells; whole-cell nystatin perforated patch-clamping; ICch | 4 μM |
Waniishi et al., 1998
|
Trachea | Cattle | Isolated smooth muscle cells and strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 30 μM |
Tao et al., 2000
|
Trachea | Dog | Isolated cultured smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 50 μM |
Mitchell et al., 2000
|
Trachea | Rabbit | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 30 μM |
Iizuka et al., 1998
|
Ureter | Guinea pig | Isolated α-toxin- or β-escin-permeabilized strips; isometric dynamometry; contractile activity | 10 μM |
Burdyga et al., 1998
|
Ureter | Rat | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 50 μM |
Burdyga et al., 2003
|
Ureter | Rat | Isolated smooth muscle cells; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 μM |
Boittin et al., 2000
|
Ureter | Rat | Isolated α-toxin- or β-escin-permeabilized strips; isometric dynamometry; contractile activity | 10 μM |
Burdyga et al., 1998
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping; IBK, ISK, ICa | 10 μM |
Herrera and Nelson, 2002
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 μM |
Rueda et al., 2002b
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell and outside-out patch-clamping; IBK | 10 μM |
Imaizumi et al., 1996
|
Urinary bladder | Guinea pig | Isolated smooth muscle cells; whole-cell amphotericin B-perforated patch-clamping, fluo-3 cellular loading, confocal fluorescence microscopy; IBK, [Ca2+]cyt imaging | 10 μM |
Herrera et al., 2001
|
Urinary bladder | Mouse | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 10 μM |
Ji et al., 2002
|
Uterus | Human | Isolated myometrial strips; indo-1 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 50 μM |
Kupittayanant et al., 2002
|
Vas deferens | Guinea pig | Isolated smooth muscle cells; fluo-4 cellular loading, confocal fluorescence microscopy; local [Ca2+]cyt imaging | 100 μM |
White and McGeown, 2003
|
Vena cava (inferior) | Rabbit | Isolated smooth muscle cells, fluo-3 cellular loading, confocal fluorescence microscopy; local [Ca2+]cyt imaging | 100 μM |
Ruehlmann et al., 2000
|