Effective concentrations of InsP3 in smooth muscle
| Tissue | Animal Species | Experimental Details (Preparation; Measurement Method; Measured Response) | Effective Concentrations of InsP3 | Reference |
|---|---|---|---|---|
| μM | ||||
| Airways | Human | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 300 | Iizuka et al., 1998 |
| Aorta | Dog | Microsomal membranes incorporated into planar lipid bilayers, microsomes; bilayer clamp amplification (for membranes), extra-microsomal anti-pyridylazo-III (for microsomes); single Ca2+ channel currents (for membranes), Ca2+ fluxes (for microsomes) | 7 (vesicles) 1 (bilayer) | Ehrlich and Watras, 1988 |
| Aorta | Guinea pig | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 100 | Nixon et al., 1994 |
| Aorta | Pig | Microsomes; extramicrosomal fluo-3, fluorometry; Ca2+ fluxes | 10 and 30 | Tovey et al., 2000 |
| Aorta | Rabbit | Isolated saponin-permeabilized smooth muscle cells; mag-fura-2 cellular loading; [Ca2+]SR, [Ca2+]mito | 100 | Gurney et al., 2000 |
| Aorta | Cattle | Purified InsP3R incorporated into planar bilayers; bilayer clamp amplification; single Ca2+ channel currents | 0.5 | Mayrleitner et al., 1991 |
| Aorta | Rat | Microsomes; 45Ca2+ loading; Ca2+ fluxes | 8 | Yusufi et al., 2002 |
| Aorta | Rat | Isolated cultured saponin-permeabilized smooth muscle cells; 45Ca2+ cellular loading; Ca2+ fluxes | 10 and 100 | Yamamoto et al., 1991 |
| Aorta | Rat | Saponin-permeabilized cultured A7r5 cell line; 45Ca2+ cellular loading; Ca2+ fluxes | 0.3-100 | Missiaen et al., 2001a |
| Cerebellum | Pig | Microsomes; extramicrosomal fluo-3, fluorometry; Ca2+ fluxes | 10 and 30 | Tovey et al., 2000 |
| Colon | Guinea pig | Isolated smooth muscle cells; fluo-3 cellular loading, wide-field fluorescent digital imaging; [Ca2+]cyt imaging | 10 | McCarron et al., 2004 |
| Colon | Guinea pig | Isolated smooth muscle cells; whole-cell patch-clamping, fluo-3 cellular loading, fluorometry or wide-field fluorescence digital imaging;ICa, [Ca2+]cyt, [Ca2+]cyt imaging | 25 (caged InsP3) | Bradley et al., 2003 |
| Colon | Guinea pig | Isolated smooth muscle cells; fluo-3 cellular loading, wide-field fluorescent digital imaging; [Ca2+]cyt imaging | 20 (caged InsP3) | McCarron and Muir, 1999 |
| Colon (circular layer) | Dog | Isolated non-inflamed and inflamed saponin-permeabilized smooth muscle cells; fura-2 cellular loading or 45Ca2+ cellular loading, cell length monitoring by phase-contrast microscope; Ca2+ fluxes, [Ca2+]cyt, contractile activity | 1 | Shi and Sarna, 2000 |
| Coronary artery | Cattle | Microsomal membranes, stripped or not of FKBP-12.6, incorporated into planar lipid bilayers; bilayer clamp amplification; single Ca2+ channel currents | 10 | Li et al., 2001 |
| Coronary artery | Cattle | Isolated α-toxin-permeabilized smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 70 | Yu et al., 2000 |
| Coronary artery | Pig | Isolated intact or saponin-permeabilized smooth muscle cells; quin-2 cellular loading, 45Ca2+ cellular loading (intact or permeabilized); cellular Ca2+ fluxes, Ca2+ fluxes from intracellular stores, [Ca2+]cyt | 3 | Ueno et al., 1987 |
| Gall bladder | Cat | Isolated saponin-permeabilized smooth muscle cells; cell length monitoring by phase-contrast microscopy; contractile activity | 1 | Yu et al., 1998 |
| Heart | Pig | Microsomes; extramicrosomal fluo-3, fluorometry; Ca2+ fluxes | 10 and 30 | Tovey et al., 2000 |
| Ileum | Guinea pig | Isolated α-toxin-permeabilized strips; isometric dynamometry; contractile activity | 300 | Iizuka et al., 1998 |
| Ileum (circular layer) | Guinea pig | Isolated β-escin-permeabilized strips; isometric dynamometry; contractile activity | 40 | Fukami et al., 1993 |
| Ileum (longitudinal layer) | Guinea pig | Isolated β-escin- or reversibly-permeabilized strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 40 | Kobayashi et al., 1989b |
| Internal anal sphincter | Rabbit | Isolated saponin-permeabilized smooth muscle cells; cell length monitoring by phase-contrast microscopy; contractile activity | 1 | Bitar et al., 1991 |
| Mesenteric artery | Rabbit | Isolated saponin-permeabilized strips; isometric dynamometry; contractile activity | 20 | Kanmura et al., 1989 |
| Mesenteric resistance arteries | Rabbit | Isolated β-escin-permeabilized smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 20 | Itoh et al., 1992 |
| Portal vein | Guinea pig | Isolated β-escin-permeabilized smooth muscle cells; furaptra cellular loading, fluorometry; [Ca2+]SR | 10 | Hirose et al., 1998 |
| Portal vein | Guinea pig | Isolated saponin- or β-escin-permeabilized strips; fluo-3 cellular loading, epifluorescence microscopy, isometric dynamometry; [Ca2+]cyt imaging, contractile activity | 25 | Somlyo et al., 1992 |
| Pulmonary artery | Cattle | Microsomes; extramicrosomal fluo-3 and fluorometry or 45Ca2+ loading; Ca2+ fluxes | 50 | Stout et al., 2002 |
| Pulmonary artery | Rabbit | Isolated β-escin- or reversibly-permeabilized strips; fura-2 cellular loading, fluorometry, isometric dynamometry; [Ca2+]cyt, contractile activity | 40 | Kobayashi et al., 1989b |
| Pulmonary artery | Rabbit | Isolated saponin- or digitonin-permeabilized strips; Ca2+-selective electrodes (extracellular), isometric dynamometry; Ca2+ efflux, contractile activity | 0.5-30 | Somlyo et al., 1985 |
| Stomach | Rabbit | Isolated saponin-permeabilized smooth muscle cells; cell length monitoring by phase-contrast microscopy; contractile activity | 1 | Murthy, 2001 |
| Stomach (antrum) | Cattle | Microsomes; extramicrosomal fluo-3 and fluorometry or 45Ca2+ loading; Ca2+ fluxes | 50 | Stout et al., 2002 |
| Stomach (antrum) | Rat | Isolated smooth muscle cells; Oregon Green BAPTA 5N cellular loading; [Ca2+]SR | 10 | White and McGeown, 2002 |
| Testicular peritubules | Rat | Isolated saponin-permeabilized smooth muscle cells; fura-2 cellular loading, fluorometry; [Ca2+]cyt | 10 | Barone et al., 2002 |
| Trachea | Cat | Isolated smooth muscle cells; whole-cell nystatin perforated patch-clamping, ICch | 200 (caged) | Waniishi et al., 1998 |
| Trachea | Cattle | Microsomal membranes incorporated into planar lipid bilayers; bilayer clamp amplification; single Ca2+ channel currents | 6 | Gaburjakova et al., 1999 |
| Trachea | Pig | Isolated smooth muscle cells; whole-cell patch-clamping; ICl(Ca) | 10 | Liu and Farley, 1996 |
| Trachea | Pig | Isolated β-escin-permeabilized smooth muscle cells; fluo-3 cellular loading, confocal fluorescence microscopy; [Ca2+]cyt imaging | 1 | Kannan et al., 1997 |
| Trachea | Rabbit | Isolated β-escin-permeabilized strips; isometric dynamometry; contractile activity | 300 | Iizuka et al., 1998 |
| Uterus (pregnant) | Rat | Isolated myometrial smooth muscle cells; 45Ca2+ cellular loading; Ca2+ efflux | 10 | Molnar and Hertelendy, 1995 |
| Vas deferens | Guinea pig | Isolated β-escin-permeabilized strips; isometric dynamometry; contractile activity | 100 | Nixon et al., 1994 |