Presence of ABC transporters in the heart detected by different molecular biology techniques
| ABC Transporter | Heart Species | Technique | Expression Levela | Reference |
|---|---|---|---|---|
| ABCB1 (MDR1 or P-gp) | Human | Slot blot hybridization | Low | Fojo et al. (1987) |
| Human | Immunohistochemistry | Very low | van der Valk et al. (1990) | |
| Human fetus | Immunohistochemistry and RNase protection | Unlikely or very low | van Kalken et al. (1992) | |
| Human | Immunohistochemistry | Low | Pavelic et al. (1993) | |
| Human | Immunohistochemistry | Intermediate | Sugawara et al. (1997) | |
| Human | RT-PCR and immunohistochemistry | Presentb | Meissner et al. (2002) | |
| Human | RT-PCR and immunohistochemistry | Presentb | Meissner et al. (2004) | |
| Human | RT-PCR | Very lowc | Nishimura et al. (2004) | |
| ABCB1 (MDR1 or P-gp) | Chinese hamster | RNase protection | Very low | Baas and Borst (1988) |
| C57Bl/6J mouse | Hydridization/Northern blot | Intermediate | Croop et al. (1989) | |
| BALB/c mouse | RNase protection | Low | Teeter et al. (1990) | |
| Wistar newborn and adult rat | RT-PCR | Low/intermediate | Cayre et al. (1996) | |
| Wistar newborn rat cultured ventricular myocytes | RT-PCR | High | Cayre et al. (1996) | |
| Sprague-Dawley rat | Western blot and immunodetection | Intermediate | Beaulieu et al. (1997) | |
| Wistar rat | Immunohistochemistry | Presentb | Estevez et al. (2000) | |
| Wistar rat | RT-PCR | Presentb | Rosati et al. (2003) | |
| FVB mouse | RT-PCR | Highc | Muramatsu et al. (2004) | |
| ABCB4 (MDR3) | Human | RNase protection | Low | Smit et al. (1994) |
| Human | Immunohistochemistry | Not detected probably due to a too low level | Smit et al. (1994) | |
| ABCB4 (MDR2) | C57Bl/6J mouse | Hydridization/Northern blot | Intermediate | Croop et al. (1989) |
| BALB/c mouse | RNase protection | Intermediate | Teeter et al. (1990) | |
| Fisher rat | RNase protection | High | Brown et al. (1993) | |
| Sprague-Dawley rat | Northern blot and PCR | Low | Furuya et al. (1994) | |
| ABCC1 (MRP1) | Human | Immunohistochemistry | High | Flens et al. (1996)d |
| CD1 mouse | Northern blot | High | Stride et al. (1996)d | |
| Human | Immunohistochemistry | Not detected | Sugawara et al. (1997)d | |
| FVB mouse | Western blot | Presentb | Wijnholds et al. (1997) | |
| Chicken | Northern blot | High | Hagen et al. (2000) | |
| Wistar rat | RT-PCR | Presentb | Rosati et al. (2003) | |
| Wistar rat | PCR | Presentb | Ghosh et al. (2004) | |
| FVB mouse | RT-PCR | Highc | Muramatsu et al. (2004) | |
| Human | RT-PCR | Intermediatec | Nishimura et al. (2004) | |
| ABCC2 (MRP2 or cMOAT) | Wistar rat | RT-PCR | Not detected | Rosati et al. (2003) |
| FVB mouse | RT-PCR | Lowc | Muramatsu et al. (2004) | |
| Human | RT-PCR | Very lowc | Nishimura et al. (2004) | |
| ABCC3 (MRP3) | FVB mouse | RT-PCR | Intermediatec | Muramatsu et al. (2004) |
| Human | RT-PCR | Lowc | Nishimura et al. (2004) | |
| ABCC4 (MRP4) | FVB mouse | RT-PCR | Intermediatec | Muramatsu et al. (2004) |
| ABCC5 (MRP5) | Human | RT-PCR and immunohistochemistry | Presentb | Dazert et al. (2003) |
| FVB mouse | RT-PCR | Intermediatec | Muramatsu et al. (2004) | |
| ABCC6 (MRP6) | FVB mouse | RT-PCR | Very lowc | Muramatsu et al. (2004) |
| ABCC10 (MRP7) | Mouse | RT-PCR and Northern blot | Presentb | Kao et al. (2002) |
| FVB mouse | RT-PCR | Intermediatec | Muramatsu et al. (2004) | |
| ABCG2 (BCRP) | C57BL/6 mouse | RT-PCR and immunohistochemistry | Presentb | Martin et al. (2004) |
| Human | RT-PCR and immunohistochemistry | Presentb | Meissner et al. (2006) | |
| ABCA1e | Human | RT-PCR | Highc | Nishimura et al. (2004) |
| ABCA5e | Mouse | Immunohistochemistry | Presentb | Kubo et al. (2005) |
| ABCA6e | Human | RT-PCR and dot blot analysis | High | Kaminski et al. (2001) |
| ABCA9e | Human | RT-PCR and dot blot analysis | High | Piehler et al. (2002) |
| ABCA8 | Human | Northern blot | High | Tsuruoka et al. (2002) |
| ABCA10e | Human | RT-PCR and dot blot analysis | High | Wenzel et al. (2003) |
| ABCD2 (ALDR)e | Human | Northern blot | High | Holzinger et al. (1997) |
cMOAT, canalicular multispecific organic anion transporter; ALDR, adrenoleukodystrophy-related
↵ a Relative because assessed by different authors
↵ b Present means that the level cannot be compared with that in other tissues because only heart was studied or very few tissues, including the heart
↵ c Assessed by the authors of this article on the basis of comparisons with other tissues analyzed
↵ d Not specified in the publication but probably corresponds to MRP1
↵ e No evidence in the literature of the role of this ABC transporter in drug disposition