TABLE 1

PDE enzyme kinetic properties

Isoform Substrate Specificity KmVmax (purified) References
cGMP cAMP cGMP cAMP
μM μmol / min / mg
PDE1A cAMP<cGMP 2.6—3.5 72.7—124 50—300 70—450 Sharma et al. (1984); Hansen et al., (1988); Sonnenburg et al. (1995); Snyder et al. (1999)
PDE1B cAMP<cGMP 1.2—5.9 10—24 30 10 Sharma and Wang, (1986); Bender et al. (2005)
PDE1C cAMP=cGMP 0.6—2.2 0.3—1.1 — — Loughney et al. (1996); Yan et al. (1996)
PDE2A cAMP=cGMP 10 30 123 120 Martins et al. (1982); Rosman et al. (1997)
PDE3A cAMP>cGMP 0.02—0.15 0.18 0.34 3.0—6 Grant and Colman (1984); Harrison et al. (1986)
PDE3B cAMP>cGMP 0.28 0.38 2.0 8.5 Degerman et al. (1987)
PDE4A cAMP>cGMP — 2.9—10 — 0.58 Wang et al. (1997); Salanova et al. (1998); Rena et al. (2001); Wallace et al. (2005)
PDE4B cAMP>cGMP — 1.5—4.7 — 0.13 Huston et al. (1997); Wang et al. (1997); Salanova et al., 1998
PDE4C cAMP>cGMP — 1.7 — 0.31 Wang et al. (1997)
PDE4D cAMP>cGMP — 1.2—5.9 — 0.03—1.56 Wang et al. (1997); Salanova et al. (1998)
PDE5A cGMP>cAMP 2.9—6.2 290 1.3 1.0 Loughney et al. (1998); Lin et al. (2000); Zoraghi et al. (2006)
PDE6A/B cGMP>cAMP 15 700 2300 — Gillespie and Beavo (1988)
PDE6C cGMP>cAMP 17 610 1400 — Gillespie and Beavo (1988)
PDE7A cAMP>cGMP — 0.1—0.2 — — Michaeli et al. (1993); Han et al. (1997)
PDE7B cAMP>cGMP — 0.03—0.07 — — Hetman et al. (2000); Sasaki et al. (2000, 2002)
PDE8A cAMP>cGMP — 0.06 — — Fisher et al. (1998)
PDE8B cAMP>cGMP — 0.10 — — Gamanuma et al. (2003)
PDE9A cGMP>cAMP 0.70—0.17 230 — — Fisher et al. (1998); Soderling et al. (1998b)
PDE10A cAMP<cGMP 13—14 0.22—1.1 — — Fujishige et al., (1999a); Kotera et al. (1999b)
PDE11A cAMP=cGMP 0.95—2.1 2.0—3.2 — — Fawcett et al. (2000); Yuasa et al. (2001b)

  • —, no reliable information available