Dose-effect relationship based on the median-effect equation for the first- and higher-order systems, using the assigned m and Dm values to calculate the dose, Dx, for different effect, (fa)xEquation 8: (D)x = Dm [(fa)/(1 - fa)]1/m, where x is the fractional inhibition as specified by fa = (fa)x. The numbers in bold are used in Fig. 11, and the symbols correspond to the same symbols used in Fig. 11.
| Assigned Dm Value for Potency | Assigned m value for Shape | Calculated Dose (D)x in μM at: | ||||||
|---|---|---|---|---|---|---|---|---|
| (D)0.1 for fa = 0.1 | (D)0.2 for fa = 0.2 | (D)0.4 for fa = 0.4 | (D)0.5 for fa = 0.5 | (D)0.6 for fa = 0.6 | (D)0.8 for fa = 0.8 | (D)0.9 for fa = 0.9 | ||
| 0.5 μM | m = 1 | 0.05555 | 0.125 | 0.3333 | 0.5 (○) | 0.75 | 2.0 | 4.5 |
| m = 3 | 0.2404 | 0.315 | 0.4368 | 0.5 | 0.5724 | 0.7937 | 1.040 | |
| m = 5 | 0.3222 | 0.3789 | 0.4611 | 0.5 | 0.5422 | 0.6598 | 0.7759 | |
| 1 μM | m = 1 (○) | 0.11111 (○) | 0.25 (○) | 0.6666 (○) | 1.0 (□) | 1.5 (○) | 4.0 (○) | 9.0 (○) |
| m = 3 (□) | 0.48075 (□) | 0.630 (□) | 0.8736 (□) | 1.0 (□) | 1.1447 (□) | 1.5874 (□) | 2.0801 (□) | |
| m = 5 (▵) | 0.6444 (▵) | 0.7579 (▵) | 0.9221 (▵) | 1.0 (▵) | 1.0845 (▵) | 1.3195 (▵) | 1.5518 (▵) | |
| 2 μM | m = 1 | 0.2222 | 0.5 | 1.3333 | 2.0 (▵) | 3.0 | 8.0 | 18.0 |
| m = 3 | 0.9615 | 1.260 | 1.747 | 2.0 | 2.2894 | 3.1748 | 4.1602 | |
| m = 5 | 1.2888 | 1.5157 | 1.8442 | 2.0 | 2.1689 | 2.6390 | 3.1037 | |
| 4 μM | m = 1 | 0.4444 | 1.0 | 2.666 | 4.0 (▿) | 6.0 | 16.0 | 36.0 |
| m = 3 | 1.9230 | 2.520 | 3.494 | 4.0 | 4.5788 | 6.3496 | 8.320 | |
| m = 5 | 2.5776 | 3.0314 | 3.688 | 4.0 | 4.3379 | 5.2780 | 6.2074 | |
| 6 μM | m = 1 | 0.6666 | 1.5 | 4.0 | 6.0 (⋄) | 9.0 | 24.0 | 54.0 |
| m = 3 | 2.8845 | 3.780 | 5.241 | 6.0 | 6.8683 | 9.5244 | 12.480 | |
| m = 5 | 3.8664 | 4.547 | 5.5326 | 6.0 | 6.5068 | 7.9170 | 9.3111 | |
| 8 μM | m = 1 | 0.8888 | 2.0 | 5.333 | 8.0 (×) | 12.0 | 32.0 | 72.0 |
| m = 3 | 3.6980 | 5.040 | 6.989 | 8.0 | 9.1577 | 12.699 | 16.641 | |
| m = 5 | 5.1552 | 6.0629 | 7.377 | 8.0 | 8.6756 | 10.556 | 12.4148 | |
| 16 μM | m = 1 | 1.7777 | 4.0 | 10.666 | 16.0 (+) | 24.0 | 64.0 | 144.0 |
| m = 3 | 7.3960 | 10.080 | 13.977 | 16.0 | 18.315 | 25.398 | 33.281 | |
| m = 5 | 10.310 | 12.126 | 14.754 | 16.0 | 17.352 | 21.112 | 24.8295 | |