TABLE 1

Specific activity and kinetic parameters for PGE2 synthases expressed in and purified from E. coli

Enzyme cDNA Source VmaxkcatKm(PGH2)kcat/Km Reference
μmol/min mg s-1 mM mM-1 s-1
mPGES-1 Human 170 50 0.16 310 Thorén et al. (2003)
mPGES-2 Monkey 3.3 1.8 0.028 65 Tanikawa et al. (2002)
cPGESa Human ≈1.9a ≈0.8b 0.014 ≈57 Tanioka et al. (2000)

  • ↵ a Multiplied by a factor of 10 since recombinant enzyme displays an order of magnitude lower activity (Tanioka et al., 2000)

  • ↵ b Calculated from Vmax