TABLE 3

Percentile distribution of amino acid residues facing lipid (out) or not facing lipid (in) in transmembrane helices The structure of each protein was viewed. Residues exposed to the surface of the protein within the transmembrane domains were considered lipid-facing (out) and those buried in between helices were considered non-lipid-facing (in). The numbers of each residue were counted. The number for each amino acid residue was calculated as follows: the sum number of each amino acid residue for six nonmitochondrial proteins was divided by the total number of all 20 amino acid residues for either in or out. The same calculation was done for the four mitochondrial proteins. The top two rows are the sum of amino acid residues from bovine rhodopsin, bovine aquaporin 0, rat Kv1.2 channel, rabbit Ca2+-ATPase, LTC4S, and FLAP. The bottom two rows are the sum of four mitochondrial respiratory chain proteins.

G A P V L I M C F Y W H K R E D Q N T S
%
In 7.3 15.3 4.2 11.7 11.1 5.6 3.1 2.3 5.2 4.4 1.5 0.8 1.2 2.3 3.8 1.7 2.9 4.0 5.4 6.1
Out 5.2 7.6 1.9 10.9 22.8 11.4 2.7 1.3 13.3 3.5 3.5 0.8 1.6 3.5 1.3 0.5 2.2 0.5 2.7 2.4
In 10.2 10.3 2.0 7.1 10.2 6.2 5.8 1.0 7.3 4.1 3.0 4.1 1.9 2.8 1.9 2.5 2.0 2.4 7.4 7.5
Out 4.7 10.3 2.7 10.7 22.8 10.3 2.7 0.8 11.1 3.9 3.3 0.9 1.9 1.9 0.6 0.2 1.4 1.2 5.8 2.7