Effects of individual chemokine receptor knockout in atherosclerosis mouse models
| Gene KO | Mouse Model | Effect | Reference |
|---|---|---|---|
| CCR1 | Knockout bone marrow transfer into LDLR deficient mice fed a high-fat diet | No effect at 8 weeks. 70% increase in lesion size in thoracic aorta at 12 weeks. | Potteaux et al., 2005 |
| Apoe−/− fed high-fat diet | Increased plaque size, T cell and IFN-γ content. | Zernecke et al., 2006 | |
| Wire injury in Apoe−/− mice | No effect | Braunersreuther et al., 2007b | |
| CCR2 | Apoe−/− fed a high-fat diet | Smaller lesions with fewer macrophages | Boring et al., 1998 |
| Ccr2−/− bone marrow transplant into ApoE3-Leiden mice fed high-fat diet | 86% reduction in lesion size | Guo et al., 2003 | |
| Transfer of Ccr2−/− bone marrow into Apoe−/− mice with established lesions | No inhibition of lesion progression compared with WT bone marrow transfer | Guo et al., 2005 | |
| CCR5 | Apoe−/− fed a chow diet | No effect on lesion size at 16 weeks | Kuziel et al., 2003 |
| Apoe−/− fed high-fat diet | Smaller lesions with fewer macrophages and T cells | Potteaux et al., 2006 | |
| Wire injury in Apoe−/− mice | Reduced neointima formation associated with increased IL-10 production | Zernecke et al., 2006 | |
| Knockout bone marrow transfer into LDLR-deficient mice fed a high-fat diet | No effect on lesion size at 8 weeks, 30% fewer macrophages. Small reduction in lesion size at 12 weeks. Increased collagen content | Braunersreuther et al., 2007b | |
| CCR6 | Apoe−/− fed high-fat diet. | 40% smaller lesions with fewer macrophages at 16 weeks, circulating inflammatory monocyte numbers reduced | Wan et al., 2011 |
| CCR7 | Apoe−/− fed a high-fat diet 16 weeks + 2 week treatment with LXR agonist to promote regression | LXR agonist induced emigration of CD68+ cells from WT plaques but no effect in CCR7 KO, i.e., regression failed. | Feig et al., 2010 |
| Ldlr−/− fed a high-fat diet | 50% smaller plaques in aorta after 12 weeks’ fat feeding, decrease in plaque macrophage numbers, increased DC and T-cell numbers, probably due to failed migration to 2° lymphoid organs. Reduced plasma IFN-γ. | Luchtefeld et al., 2010 | |
| Apoe−/− fed a high-fat diet for 16 weeks followed by 4 week statin treatment | Statins-induced emigration of CD68+ cells from WT plaques but not in CCR7 KO. | Feig et al., 2011b |