Release of opioid factor with incubation of brain tissue
Rat brain homogenates were centrifuged, resuspended, and incubated at the indicated temperature/time. The soluble extracts were then tested in a [3H]naloxone binding assay and the inhibition determined. From Pasternak et al. (1975c).
| Addition | [3H]Naloxone Binding | |
|---|---|---|
| cpm | Inhibition | |
| % | ||
| None | 4142 | |
| 0° Supernatant, 40 min | 3154 | 24 |
| 25° Supernatant, 40 min | 1905 | 54 |
| 37° Supernatant, 40 min | 1493 | 64 |
| 37o Supernatant, 120 min | 1166 | 72 |