Regulation of ROCK activity
| Mechanism | Effect | ROCK1 | ROCK2 | Reference |
|---|---|---|---|---|
| Binding to RBD | Stimulation | RhoA | RhoA | Amano et al., 1999 |
| RhoB | RhoB | |||
| RhoC | RhoC | |||
| Binding to PH domain | Stimulation | PIP2, PIP3 | PIP2, PIP3 | Yoneda et al., 2005; Wen et al., 2008 |
| AA | AA | Feng et al., 1999 | ||
| Binding to N terminus | Inhibition | RhoE | — | Riento and Ridley, 2003 |
| Binding | Stimulation (prevents RhoE binding) | PDK1 | — | Pinner and Sahai, 2008 |
| Binding | Inhibition | Gem | Rad | Ward et al., 2002 |
| Autophosphorylation | Activation marker | Ser1333 | Ser1366 | Chuang et al., 2012 |
| Phosphorylation | Inhibition | — | Tyr722 (Src) | Lee et al., 2010 |
| Stimulation (potentiates RhoA effect) | Multiple site (polo-like kinase 1) | Lowery et al., 2007 | ||
| Cleavage of C terminus | Stimulation | Caspase3 | Granzyme B | Sebbagh et al., 2001, 2005 |
| Caspase2 | Sapet et al., 2006 |
AA, arachidonic acid; PDK1, phosphoinositide-dependent kinase-1; PH, pleckstrin homology; PIP2, phosphatidylinositol-4,5-bisphosphate; PIP3, phosphatidylinositol-(3,4,5)-trisphosphate.