Expression of mAC isoforms at the mRNA level
Expression pattern of AC isoforms in rodent brain and human tissue. Sanabra and Mengod (2011) used in situ hybridization to examine and compare the AC isoform expression in rodent brain tissues. Semiquantitative RT-PCR was used to examine and compare the AC isoform expressions in 16 human tissues (Ludwig and Seuwen, 2002).
| AC Isoform | Rodent CNS Expression (Sanabra and Mengod, 2011) | Relative Human Tissue Abundance (Ludwig and Seuwen, 2002) |
|---|---|---|
| AC1 | Hippocampus, cerebellum, cortex, and thalamus | Peripheral blood leukocytes > heart ∼ skeletal muscle ∼ kidney ∼ pancreas ∼ spleen ∼ testis ∼ ovary |
| AC2 | Limbic and widespread | Skeletal muscle ∼ testis > heart ∼ prostate ∼ ovary ∼ colon |
| AC3 | Widespread | Placenta ∼ testis ∼ ovary ∼ colon > lung ∼ spleen ∼ thymus |
| AC4 | Not detected | Heart ∼ placenta ∼ lung ∼ spleen ∼ testis ∼ ovary ∼ small intestine |
| AC5 | Caudate putamen and olfactory tubercle | Testis ∼ heart ∼ prostate ∼ ovary ∼ small intestine ∼ colon |
| AC6 | Widespread | Heart ∼ kidney ∼ prostate ∼ testis ∼ ovary ∼ small intestine ∼ colon |
| AC7 | Thalamus and hypothalamus | Peripheral blood leukocytes > placenta ∼ lung ∼ spleen ∼ thymus > heart ∼ liver ∼ ovary ∼ colon |
| AC8 | Hippocampus, cerebellum, cortex, thalamus and hypothalamus | Testis (limited detection) |
| AC9 | Cortex and hippocampus | Heart ∼ placenta ∼ lung ∼ liver ∼skeletal muscle ∼ kidney ∼ pancreas ∼ testis ∼ ovary ∼ peripheral blood leukocytes |