TABLE 3

CB, FFA, and prostanoid receptor fluorescent ligands

Tool	Data	Reference
CB₁ Receptor and GPR55 Receptor Ligands
T1117	hGPR55-mediated Ca²⁺ response in HEK293 cells^{^a}	Daly et al., 2010
	mCB₁ 10% displacement of [³H]CP-55,940 with 1 µM T1117	Daly et al., 2010
	hCB₁ K_d = 460 ± 80 nM	Bruno et al., 2014
CB Receptor Ligands
2-AGE-biotin-3b^{^b}	hCB₁ K_i = 221 ± 8 nM	Martin-Couce et al., 2011
2-AGE-biotin-3b^{^b}	hCB₂ K_i = 450 ± 11 nM	Martin-Couce et al., 2011
NMP6	hCB₁ < 40% at 10 µM	Petrov et al., 2011
NMP6	hCB₂ K_i = 387 nM	Petrov et al., 2011
Biotin-HU210-1^{^a}	hCB₁ K_i = 2.4 ± 0.4 nM	Martín-Couce et al., 2011
Biotin-HU210-1^{^a}	hCB₂ K_i = 1.6 ± 0.4 nM	Martín-Couce et al., 2011
Biotin-HU308-3^{^a}	hCB₁ K_i > 5000 nM	Martín-Couce et al., 2011
Biotin-HU308-3^{^a}	hCB₂ K_i = 44 ± 4 nM	Martín-Couce et al., 2011
NIR-mbc94	mCB₂ Ki = 260 nM	Sexton et al., 2011
NIR760-mbc94	mCB₂ K_d = 26.9 ± 3.7 nM	Zhang et al., 2013
NIR760-Q	hCB₂ K_d = 75.51 ± 27.97 nM	Wu et al., 2014b
ZW760-mbc94	mCB₂ K_d = 53.9 ± 13.0 nM	Wu et al., 2014a
NIR760-XLP6	mCB₁ K_d > 10,000 nM	Ling et al., 2015
NIR760-XLP6	mCB₂ K_d = 169.1 ± 66.09 nM	Ling et al., 2015
IR700DX-mbc94	mCB₂ K_d = 42.0 ± 19.6 nM	Zhang et al., 2014
FFA Receptor Ligands
TUG-905-NBD-4	hFFA1 K_d = 7.4 ± 1.3 nM	Christiansen et al., 2016
F-TAK-875A	hFFA1 K_d = 15.1 ± 3.2 nM	Ren et al., 2016
TAK-875-Alexa488-16	hFFA1-mediated Ca²⁺ response equipotent to TAK-875	Bertrand et al., 2016b
EP₃ Receptor Ligands
Pyryllium-labeled cinnamon acid derivative 8	hEP₁ Ki = 68,625 ± 12,735 nM	Tomasch et al., 2012
	hEP₂ Ki = 2943 ± 279 nM
	hEP₃ Ki = 135 ± 23 nM
	hEP₄ Ki = 902 ± 226 nM

↵^a HEK293 cells stably expressing GPR55.
↵^b Biotin-tagged ligand (precursor for fluorescent tool) that conjugates with streptavidin-fluorophore in situ during the biologic experiment to provide corresponding fluorescent tool.