TABLE 3

Cellular proteasome substrates

SubstrateUb/PathwayLocalizationt1/2Reference
GFPuYes/CL1 degron30 minBence et al. (2001)
 NLS-GFPuYes/CL1 degronNuclear60 minBennett et al. (2005)
 NES-GFPuYes/CL1 degronCytosolic60 minBennett et al. (2005)
 PSD95-GFPuYes/CL1 degronPostsynapticNDWang et al. (2008)
 SNAP25-GFPuYes/CL1 degronPresynapticNDWang et al. (2008)
TCR-α–GFPYes/ERADNDDeLaBarre et al. (2006)
Ub-M-GFPYes/“normal”“Stable”Dantuma et al. (2000)
Ub-R-GFPYes/N-end rule“Short”Dantuma et al. (2000)
UbG76V-GFPYes/UFD“Short”Dantuma et al. (2000)
UbG76V-dendra2Yes/UFDNDHamer et al. (2010)
GFP-ODCNo2 hLi et al. (1998)
  • M, methionine; ND, not determined; NES, nuclear export signal; NLS, nuclear localization signal; PSD95, postsynaptic density 95; R, arginine; SNAP25, synaptosomal-associated protein 25; t1/2, in vivo half-life; TCR-α, T-cell receptor α chain.