Biochemical and Biophysical Research Communications
Regular ArticleESR Spin Trapping Investigation on Peroxynitrite Decomposition: No Evidence for Hydroxyl Radical Production
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Effect of dielectric barrier discharge plasma on persulfate activation for rapid degradation of atrazine: Optimization, mechanism and energy consumption
2022, Environmental ResearchCitation Excerpt :During discharge, an obvious signal appeared, except for the fourfold peak of conventional DMPO-OH (1:2:2:1), the triplet in the EPR spectrum belonging to 5,5-dimethyl-2-oxopyrroline-1-oxyl (DMPOX). Active substances such as RNS and O2 (a1Δ) existing in the discharge system interact with DMPO to form C(OO•)-N•(O), and the double free radical will rearrange into strongly oxidizing nitrogen-like groups and generate DMPO-O2H and DMPOX after a redox reaction with DMPO (Bilski et al., 1996; Liu et al., 2016; Shi et al., 1994). In addition, nitro radicals or peroxynitrite affect the signal peaks, causing interference with the signals of DMPO-OH and DMPO-SO4 (Wang et al., 2018).
Roles of ROS, Nrf2, and autophagy in cadmium-carcinogenesis and its prevention by sulforaphane
2018, Toxicology and Applied PharmacologyCitation Excerpt :The ROS generation decreased when catalase, which is a specific antioxidant against H2O2, was added to cadmium-treated BEAS-2BR cells (Fig. 1A). We also measured the oxygen radical levels in cadmium-treated BEAS-2BR cells and the possible inhibition by an antioxidant using ESR spin trapping according to the method described previously (Shi et al., 1994a; Shi et al., 1994b). This method involves the addition-type reaction of a short-lived radical with a paramagnetic compound (spin trap) to form a relatively long-lived free radical product (spin adduct), which can then be studied using ESR.
Elimination of chlorine-refractory carbamazepine by breakpoint chlorination: Reactive species and oxidation byproducts
2018, Water ResearchCitation Excerpt :Similar results were found in a previous study (Ratnayake Bandara et al., 1992), and the signal was attributed to the 2-(N-chloroimino)-5,5-dimethylpyrrolidine-l-oxyl generated via the reaction between the nitroxyl radical and DMPO. Shi et al. (1994) observed similar electron spin resonance spectra and attributed it to the signals of 5,5-dimethylpyrrolidine-2-oxyl generated via the reaction between peroxynitrite and DMPO. However, a DMPO–OH signal generated from OH and DMPO was not observed, because the OH concentration was too low to generate DMPO-OH or the generated DMPO–OH at a low concentration was destroyed by nitroxyl radicals or peroxynitrite.
7-Hydroxycoumarin modulates the oxidative metabolism, degranulation and microbial killing of human neutrophils
2013, Chemico-Biological InteractionsCitation Excerpt :We used DMPO and 4-POBN as spin traps to detect the coumarin free radical intermediates produced by MPO. The peroxidase-catalyzed oxidation of the hydroxylated coumarins (1 or 2) in the presence of DMPO gave the typical nine-line spectrum of 5,5-dimethyl-1-pyrrolidone-2-oxyl-1 (DMPOX), whose characteristic hyperfine coupling constants aN = 7.2 G and aH = 4.1 G, appeared at g = 2.0085, agreeing with literature values [39–41] and the corresponding simulated spectra (Fig. 5A). The acetoxylated coumarins (3 or 4) did not produce a significant EPR signal in the presence of HRP-H2O2 and DMPO under the assessed conditions.
Chain scission of hyaluronan by peroxynitrite
2003, Archives of Biochemistry and BiophysicsNitric oxide enhances MPP<sup>+</sup>-induced hydroxyl radical generation via depolarization activated nitric oxide synthase in rat striatum
2001, Brain ResearchCitation Excerpt :Intracellular Ca2+ overload is then considered to cause cell death under physiological conditions. Although O2− and NO are known to rapidly react to form the stable peroxynitrite (ONOO−) and its composition generates to ·OH [1,21], these data are disputed [5]. Although the precise mechanism of ·OH generation by the action of MPP+ is obscure, we previously found a concomitant increase of DA and ·OH generation on MPP+-treated animals [16].