Regular Article
The Human Bradykinin B2 Receptor Gene: Full-Length cDNA, Genomic Organization and Identification of the Regulatory Region

https://doi.org/10.1006/bbrc.1995.1800Get rights and content

Abstract

Hybridization of Northern blots with a probe corresponding to the coding region of the bradykinin B2 receptor revealed a single transcript of approximately 4.0 kb. Using the same probe, we detected a 2.6 kb human cDNA clone that overlapped 103 bp with the 3′ end of the known coding region and contained a classical polyadenylation site and a polyA tail. The gene for the human B2 receptor was isolated from a human placenta genomic library. Analysis of several genomic λ clones indicated that the B2 receptor gene is organized in three exons. Genomic Southern analysis revealed the B2 receptor to be encoded by a single copy gene. In situ hybridization showed that the gene is located on chromosome 14q32. By testing different putative regulatory fragments in a luciferase reporter assay, a gene activating function of the 5′ upstream region of exon 1 was demonstrated.

References (0)

Cited by (70)

  • G Protein-Coupled Kinin Receptors and Immunity Against Pathogens

    2017, Advances in Immunology
    Citation Excerpt :

    In the early 1980s, Regoli and Barabé (1980) distinguished the existence of two kinin receptors based on differences of agonist potencies and synthesis of subtype-specific synthetic antagonists further validated their predictions (Vavrek & Stewart, 1985). Gene cloning in the 1990s led to the characterization of kinin receptors as members of the large rhodopsin family of GPCRs containing seven-membrane-spanning domains (Hess, Borkowski, Young, Strader, & Ransom, 1992; Kammerer, Braun, Arnold, & Roscher, 1995; McEachern et al., 1991; McIntyre, Phillips, Skidmore, Brown, & Webb, 1993; Pesquero et al., 1994, 1996). Insight on the functional role of B2R and B1R came with the development of knockout mice for each subtype of kinin receptors (Borkowski et al., 1995; Cayla et al., 2007; Pesquero et al., 2000).

  • Acute adverse effects of angiotensinconverting-enzyme inhibitors

    2007, Bulletin de l'Academie Nationale de Medecine
  • The Influence of Gender on Cough Reflex Sensitivity

    2004, Principles of Gender-Specific Medicine
View all citing articles on Scopus
View full text