Identification of 6H1 as a P2YPurinoceptor: P2Y5

doi:10.1006/bbrc.1996.0189

Biochemical and Biophysical Research Communications

Volume 219, Issue 1, 6 February 1996, Pages 105-110

https://doi.org/10.1006/bbrc.1996.0189 Get rights and content

Abstract

We have determined the identity of the orphan G-protein coupled receptor cDNA, 6H1, present in activated chicken T cells, as a subtype of P_2Ypurinoceptor. This identification is based first on the degree of sequence identity shared with recently cloned members of the P_2Yreceptor family and second on the pharmacological profile. Upon transient expression in COS-7 cells the 6H1 receptor bound the radiolabel [³⁵S]dATPαS specifically and with high affinity (K_d, 10 nM). This specific binding could be competitively displaced by a range of ligands active at P₂purinoceptors, with ATP being the most active (K_i, 116 nM). Such competition studies have established the following rank order of activity: ATP ADP 2-methylthioATP α,β-methyleneATP, UTP, thus confirming 6H1 as a member of the growing family of P_2Ypurinoceptors. As the fifth receptor of this type to be identified we suggest that it be named P2Y₅.

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^☆: $Abbreviations:$ 2-MeSATP, 2-methylthioATP; α,β-meATP, α,β-methyleneATP; DMEM, Dulbecco's modified Eagle medium; EST, expressed sequence tag; TM, transmembrane.

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Regular ArticleIdentification of 6H1 as a P2YPurinoceptor: P2Y5☆

Abstract

Regular Article
Identification of 6H1 as a P_2YPurinoceptor: P2Y₅☆