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Cloning of a Novel Member of the G Protein-Coupled Receptor Family Related to Peptide Receptors,☆☆

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Abstract

We have used PCR with degenerate oligonucleotide primers to clone novel members of the G protein-coupled receptor (GPCR) superfamily. We report here a novel gene,CEPR,which encodes a candidate receptor that is most similar to the peptide receptor family. The coding region of the humanCEPRgene predicts a seven transmembrane domain (TM) receptor of 375 amino acids. CEPR has 28–30 percent amino acid identity to angiotensin II and interleukin 8 receptors, and slightly lower percent identity to many other GPCRs. Northern blot analysis reveals a 3.3 kb CEPR transcript in different regions of human brain and in various peripheral tissues. The ubiquitous tissue distribution of CEPR, its expression in early development, and its conservation in evolution indicate a potentially important biological function for this receptor and its putative peptide ligand.

References (11)

  • P. Gregor et al.

    FEBS Lett.

    (1996)
  • P. Gregor et al.

    J. Biol. Chem.

    (1996)
  • B.F. O'Dowd et al.

    J. Biol. Chem.

    (1989)
  • S. Watson et al.

    The G-Protein Linked Receptor Factsbook

    (1994)
  • D. Richter et al.

    J. Receptor Res.

    (1991)
There are more references available in the full text version of this article.

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Abbreviations: PCR, polymerase chain reactionGPCR, G protein-coupled receptor; kb, kilobases; bp, base pair; TM, transmembrane domain;

☆☆

The nucleotide sequence reported in this paper has been deposited with Genbank/EMBL Data Libraries under accession number U77827.

2

To whom correspondence should be addressed at Institute for Metabolic Disorders, B-24, Bayer Corp., 400 Morgan Lane, West Haven, CT 06516, USA. E-mail: [email protected]; Fax: (203) 937-2686.

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