Regular Article
Stimulation of Membrane-Bound Guanylate Cyclase Activity by 17-β Estradiol

https://doi.org/10.1006/bbrc.1998.9716Get rights and content

Abstract

Estrogen is known to exert both long-term genomic and short-term nongenomic actions. The long-term genomic actions of estrogen are mediated by the binding of estrogen to its cytoplasmic/nuclear receptor that subsequently triggers the transcription of target genes. In contrast, little is known about the molecular basis of the nongenomic actions of estrogen. We have found that 17-β estradiol activates membrane-bound guanylate cyclase and enhances atrial natriuretic factor (ANF)-stimulated guanylate cyclase activity in PC12 cell membranes. Membrane-bound guanylate cyclase, GC-A, contains an extracellular ANF-binding domain and an intracellular kinase-like domain plus catalytic domain. The kinase-like domain plus the catalytic domain of guanylate cyclase (GC-kc) can be expressed in E. Coli and functionally renatured. 17-β estradiol also activates the bacteria expressed GC-kc, indicating that 17-β estradiol can directly interact with membrane-bound guanylate cyclase. These results demonstrate that 17-β estradiol exerts a non-genomic action on membrane-bound guanylate cyclase.

References (20)

  • I. Nemere et al.

    Biochem. Biophysics. Res. Commun.

    (1998)
  • D.G. Garbers et al.

    J. Biol. Chem.

    (1994)
  • C.-H. Chang et al.

    J. Biol. Chem.

    (1993)
  • T. Duda et al.

    FEBS Lett.

    (1993)
  • M.J. Hickey et al.

    Gynecology and Obstetrics

    (1991)
  • C.A. Mashchak et al.

    J. Reprod. Med.

    (1985)
  • M.G. Crane et al.

    Ann. Intern. Med.

    (1971)
  • B.S. McEwen

    Trends Pharmacol. Sci.

    (1991)
  • R.T. Moss et al.

    Recent Progr. Hormone Res.

    (1997)
There are more references available in the full text version of this article.

Cited by (0)

Sciarra, J. J.

1

To whom all correspondence should be addressed at Department of Medicine, Division of Hypertension, Case Western Reserve University School of Medicine, W 165, Cleveland, OH 44106. Fax: (216) 368-4752. E-mail:[email protected].

View full text