Exercise Decreases Cytosolic Aconitase Activity in the Liver, Spleen, and Bone Marrow in Rats

doi:10.1006/bbrc.2001.4536

Biochemical and Biophysical Research Communications

Volume 282, Issue 1, 23 March 2001, Pages 264-267

https://doi.org/10.1006/bbrc.2001.4536 Get rights and content

Abstract

Effects of strenuous exercise on cytosolic aconitase activity (CAA) were investigated in this study. Female Sprague-Dawley rats were randomly assigned to four groups: S1 (Sedentary), S2 (Sedentary + L-NAME [N-nitro- $l$ -arginine methyl ester]), E1 (Exercise), and E2 (Exercise + L-NAME). Rats in the E1 and E2 groups swam for 2 h/day for 3 months. L-NAME (an inhibitor of NOS) in drinking water (1 mg/ml) was administered to rats in the S2 and E2 groups for the same period. At the end of the third month, the CAA in the liver, spleen, and bone marrow cells was measured. In the exercise group (E1), CAA in the liver, spleen, and bone marrow cells was 19.99 ± 1.49, 1.61 ± 0.13, and 0.59 ± 0.09 mU/mg protein, respectively. These values were significantly lower than the corresponding sedentary values in the S1 group (33.96 ± 1.38, 3.96 ± 0.19, and 3.20 ± 0.18 mU/mg protein) (P < 0.01, 0.001, and 0.001, respectively). The treatment of L-NAME led to a significant increase in tissue CAA in the sedentary rats (S2). Also, the significantly higher CAA in the liver, spleen, and bone marrow cells was found in the exercised rats treated with L-NAME (E2) (29.50 ± 1.27, 2.89 ± 0.25, and 1.34 ± 0.20 mU/mg) than without L-NAME (E1) (P < 0.01, 0.01, 0.05, respectively). However, the values in the E2 group were still significantly lower than those in the S1 group (P < 0.05, 0.01, and 0.001, respectively). This indicates that L-NAME treatment can partly recover the decreased CA in tissues in the exercised rats. These results provide evidence for the existence of the increased activity of IRP1 (iron regulatory protein 1) that is probably induced by the increased nitric oxide production in the strenuously exercised rats.

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Citation Excerpt :
There are data indicating that NO is generated in skeletal [46] and cardiac [20] muscles in response to exercise. Furthermore, indirect [47] and direct [48] evidence is available for exercise-induced increased NO production in liver. On the other hand, we evaluated respiration rates in isolated preparations, and this excludes the possibility that the low value they exhibit in HE group is due to a NO-dependent inhibition of cytochrome oxidase, which is largely competitive with oxygen [49].
We investigated effects of prolonged aerobic exercise and severe hyperthyroidism on indices of oxidative damage, susceptibility to oxidants, and respiratory capacity of homogenates from rat liver, heart and skeletal muscle. Both treatments induced increases in hydroperoxide and protein-bound carbonyl levels. Moreover, the highest increases were found when hyperthyroid animals were subjected to exercise. These changes, which were associated to reduced exercise endurance capacity, were in part due to higher susceptibility to oxidants of hyperthyroid tissues. Levels of oxidative damage indices were scarcely related to changes in antioxidant enzyme activities and lipid-soluble antioxidant concentrations. However, the finding that, following exercise the scavenger levels generally decreased in liver homogenates and increased in heart and muscles ones, suggested a net shuttle of antioxidants from liver to other tissues under need. Aerobic capacity, evaluated by cytochrome oxidase activity, was not modified by exercise, which, conversely, affected the rates of oxygen consumption of hyperthyroid preparations. These results seem to confirm the higher susceptibility of hyperthyroid tissues to oxidative challenge, because the mechanisms underlying the opposite changes in respiration rates during State 4 and State 3 likely involve oxidative modifications of components of mitochondrial respiratory chain, different from cytochrome aa₃.
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Regular ArticleExercise Decreases Cytosolic Aconitase Activity in the Liver, Spleen, and Bone Marrow in Rats

Abstract

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Biochim. Biophys. Acta

Arch. Biochem. Biophys.

J. Biol. Chem.

J. Biol. Chem.

The increased expression of transferrin receptor on the membrane of erythroblast in strenuously exercised rats

J. Appl. Physiol.

Negative correlation of plasma nitric oxide (NO) and iron levels in the exercised rats

Mol. Cell. Biochem.

The increased nitric oxide is one of the causes for the changes in iron metabolism in the exercised rats

Am. J. Physiol.

Regular Article
Exercise Decreases Cytosolic Aconitase Activity in the Liver, Spleen, and Bone Marrow in Rats