Regular ArticleCell Cycle-Dependent Nuclear Localization of Exogenously Added Fibroblast Growth Factor-1 in BALB/c 3T3 and Human Vascular Endothelial Cells
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Intracellular partners of fibroblast growth factors 1 and 2 - implications for functions
2021, Cytokine and Growth Factor ReviewsCitation Excerpt :Upon binding and activation of their specific receptors (FGFRs) they stimulate intracellular signaling pathways such as PKC/PLCγ, PI3K/Akt, and Ras/MAPK [2,3]. In addition to the signaling through the tyrosine kinase receptors, FGF1 and FGF2 have been shown to translocate across cellular membranes and reach the cytosol and nucleus, an unusual feature for growth factors [4–6]. Even though this characteristic is shared by these two FGFs, most of the studies on the intracellular fate have focused on FGF1, the best-studied member of canonical FGFs.
Translocation of Exogenous FGF1 and FGF2 Protects the Cell against Apoptosis Independently of Receptor Activation
2018, Journal of Molecular BiologyCitation Excerpt :This discrepancy may arise from the fact that the conditions of experiment are very harsh for the cells, that is, starvation and the presence of FGFR inhibitor and two other compounds (Suppl. Fig. 5c). Despite the fact that the translocation of FGF1 and FGF2 is a well-known phenomenon [7,9,18,37], we performed a control experiment to confirm that in the conditions used throughout the experiments FGF1 and FGF2 were indeed translocated into the cell upon serum-starvation (Fig. 4c). Using cell fractionation [22], we showed that in starved NIH 3T3 cells exogenously added FGF1 and FGF2 were found in the cytosolic (C) and nuclear (N) fractions in the absence or presence of an FGFR inhibitor (PD173074) (Fig. 4c).
Translocation of exogenous FGF1 into cytosol and nucleus is a periodic event independent of receptor kinase activity
2011, Experimental Cell ResearchCitation Excerpt :It has previously been described that serum deprivation is required for efficient translocation into the cells, and that the translocation of FGF1 into cytosol and nucleus is delayed by 2–4 h compared to its endocytic uptake [15,20]. Also, a correlation between FGF1/FGF2 translocation and the G1 phase of the cell cycle has been observed, and it was therefore suggested that the translocation is somehow linked or restricted to G1 [20–23]. In this paper we have investigated the timing of the FGF1 translocation and the role of FGFR1 signalling in this process.
Post treatment with an FGF chimeric growth factor enhances epithelial cell proliferation to improve recovery from radiation-induced intestinal damage
2010, International Journal of Radiation Oncology Biology PhysicsIncreased protein stability of FGF1 can compensate for its reduced affinity for heparin
2009, Journal of Biological Chemistry