Regular Article
Expression of Lumican in Thickened Intima and Smooth Muscle Cells in Human Coronary Atherosclerosis

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Abstract

Lumican is a member of a small leucine-rich proteoglycan family. Members of this family play an important role in cell migration and proliferation during embryonic development, tissue repair, and tumor growth. Lumican is reported to be overexpressed during the wound-healing process in the cornea and ischemic and reperfused heart. Recently, we found that lumican mRNA and its protein are expressed in cultured vascular smooth muscle cells (VSMCs) from the rat aorta. However, the expression and role of lumican in human atherosclerotic tissues are not clearly elucidated. In the present study, we aimed to clarify whether lumican is expressed in VSMCs and its localization in human coronary atherosclerotic tissues. The lumican protein and its mRNA were expressed in a small number of VSMCs in the media of normal coronary artery, but the lumican protein was not localized in the medial stroma. In contrast, the lumican protein and its mRNA were expressed in most of VSMCs that migrated into the thickened intima, but not in infiltrating foamy macrophages. The lumican protein was prominently localized in the thickened intimal stroma. The lumican protein and its mRNA were also expressed in VSMCs in the inner layer of the media and its protein was localized in medial stromal tissues. These findings indicate that the lumican protein is mainly synthesized by intimal and medial VSMCs in coronary atherosclerosis and that lumican contributes to collagen fibrillogenesis of coronary atherosclerosis.

References (33)

  • S. Saika et al.

    Role of lumican in the corneal epithelium during wound healing

    J. Biol. Chem.

    (2000)
  • E. Schonherr et al.

    Interaction of biglycan with type I collagen

    J. Biol. Chem.

    (1995)
  • K.G. Vogel et al.

    The effect of proteoglycans on the morphology of collagen fibrils formed in vitro. Collagen

    Relat. Res.

    (1987)
  • T.N. Wight et al.

    The role of proteoglycans in cell adhesion, migration and proliferation

    Curr. Opin. Cell. Biol.

    (1992)
  • H. Baba et al.

    Expression and localization of lumican in the ischemic and reperfused rat heart

    Jpn. Circ. J.

    (2001)
  • T.C. Blochberger et al.

    cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine

    J. Biol. Chem.

    (1992)
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    1

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