Elsevier

Genomics

Volume 45, Issue 3, 1 November 1997, Pages 607-617
Genomics

Regular Article
Identification of a Gene (GPR30) with Homology to the G-Protein-Coupled Receptor Superfamily Associated with Estrogen Receptor Expression in Breast Cancer,☆☆,

https://doi.org/10.1006/geno.1997.4972Get rights and content
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Abstract

Using the technique of differential cDNA library screening, a cDNA clone was isolated from an estrogen receptor (ER)-positive breast carcinoma cell line (MCF7) cDNA library based upon the overexpression of this gene compared to an ER-negative cell line (MDA-MB-231). Sequence analysis of this clone determined that it shared significant homology to G-protein-coupled receptors. This receptor, GPCR-Br, was abundantly expressed in the ER-positive breast carcinoma cell lines MCF7, T-47D, and MDA-MB-361. Expression was absent or minimal in the ER-negative breast carcinoma cell lines BT-20, MDA-MB-231, and HBL-100. GPCR-Br was ubiquitously expressed in human tissues examined but was most abundant in placenta. GPCR-Br expression was examined in 11 primary breast carcinomas. GPCR-Br was detected in all 4 ER-positive tumors and only 1 of 7 ER-negative tumors. Based upon PCR analysis in hybrid cell lines, the gene for GPCR-Br (HGMW-approved symbol GPR30) was mapped to chromosome 7p22. The pattern of expression of GPCR-Br indicates that this receptor may be involved in physiologic responses specific to hormonally responsive tissues.

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The nucleotide sequence for the GPCR-Br cDNA and for the 5′ flanking region published in this paper have been submitted to the GenBank/EMBL database at NCBI and assigned Accession Nos. U63917 and AF007171, respectively. In addition, GPCR-Br has been submitted to the G Protein-Coupled Receptor Database [http://recep tor.mgh.harvard.edu/GCRDBHOME.html] and has been assigned the symbol GPR30 by Dr. L. E. Kolakowski, Jr.

☆☆

ER, estrogen receptorRT, reverse transcriptase; GPCR, G-protein-coupled receptor(s); ATII 1A R, angiotensin II 1A receptor (SWISS-PROT Accession No. P30556); IL-8A R, interleukin 8A receptor (SWISS-PROT Accession No. P25024); CK R1, chemokine receptor type I (SWISS-PROT Accession No. P32246); RH, radiation hybrid; NCBI, National Center for Biotechnology Information; TM, transmembrane;

F. Bresciani, Ed.

1

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