Elsevier

Journal of Autoimmunity

Volume 11, Issue 5, October 1998, Pages 395-402
Journal of Autoimmunity

Regular Article
Role of IL-10 in the Abnormalities of Early Cell Activation Events of Lymphocytes from Patients with Systemic Lupus Erythematosus

https://doi.org/10.1006/jaut.1998.0228Get rights and content

Abstract

Lymphocytes from patients with systemic lupus erythematosus (SLE) exhibit abnormalities in early cell activation events as well as increased production of IL-10. We explored the possible role of IL-10 on defective cell activation events of SLE lymphocytes and first studied thein vitroeffect of IL-10 on peripheral blood mononuclear cells (PBMNC) from healthy subjects. After 5 days of culture in the presence of exogenous IL-10, these cells demonstrated abnormal expression of CD69 as well as high intracellular pH and defective activation of the Na+/H+anti-porter by PMA. We then investigated the effect of IL-10 blockade on PBMNC from SLE patients. SLE PBMNC cultured for 5 days with a blocking anti-IL-10 monoclonal antibody (mAb) partially corrected abnormalities in CD69 expression and intracellular pH; however, in 1/5 patients studied, no significant positive effect was observed. The effect of the anti-IL-10 mAb was apparently not related to protection against activation-induced cell death. We conclude that IL-10 in normal PBMNC induce some of the defects in early cell activation events seen in SLE lymphocytes. Accordingly, the blockade of IL-10 partially corrects these abnormalities in SLE cells. Our data further support the importance of IL-10 in immune dysfunction seen in SLE.

References (0)

Cited by (16)

View all citing articles on Scopus
f1

Correspondence to: Dr Roberto González-Amaro, Departamento de Inmunologı́a, Facultad de Medicina, UASLP, Ave. V. Carranza 2405, 78210 San Luis Potosı́, S.L.P., México. Fax: 52 48 177706. E-mails:[email protected]@pasteur.fmed. uaslp.mx

View full text