Summary
The aim of the present investigation was to examine whether or not presynaptic facilitatory β-adrenoceptors are detectable on the postganglionic nerves in the rabbit isolated ear artery. Strips of rabbit central ear artery were incubated with 3H-noradrenaline (10−7 mol/l; 30 min or 10−6 mol/l; 60 min). Subsequently, they were washed repeatedly with physiological salt solution. The strips were subjected to electrical-field stimulation (S1–S8) and the resultant 3H-overflow was determined.
When the ear artery was stimulated with 150 pulses (0.5 ms; 3 Hz; 225 mA), isoprenaline (10−9−10−6 mol/l) either alone or in the presence of either rauwolscine (10−6 mol/l) or phentolamine (10−6 mol/l) did not alter the stimulation-evoked 3H-overflow. This was also the case in the presence of rauwolscine (10−6 mol/l) plus either the selective phosphodiesterase inhibitor ICI 63 197 (3 × 10−5 mol/l) or forskolin (10−6 mol/l). When the ear artery was stimulated with 300 pulses (1 ms; 5 Hz; 225 mA), isoprenaline had no effect on the stimulation-evoked 3H-overflow. This was also the case when phentolamine (10−6 mol/l) was present. Propranolol (10−7−10−5 mol/l) did not alter the stimulation-evoked 3H-overflow. In some experiments, the stimulation current was reduced to 175 mA in order to obtain similar reference release (S3) values despite the presence of rauwolscine (150 pulses; 0.5 ms; 3 Hz). Even then, isoprenaline (10−9−10−6 mol/l) did not change stimulation-evoked 3H-overflow. The results suggest that postganglionic sympathetic nerves in rabbit central ear artery do not possess presynaptic facilitatory β-adrenoceptors.
Similar content being viewed by others
References
Abrahamsen J, Nedergaard OA (1988) Modulation of stimulation-evoked [3H]-noradrenaline release from rabbit isolated ear artery. Br J Pharmacol 94: 328P
Abrahamsen J, Nedergaard OA (1991) Presynaptic modulation by adrenaline of 3H-noradrenaline release in rabbit ear artery. Pharmacology, in press
Endo T, Starke K, Bangerter A, Taube HD (1977) Presynaptic receptor systems on the noradrenergic neurones of the rabbit pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 296:229–247
Enero MA (1979) Neurotransmission in rat portal vein. In: Langer SZ, Starke K, Dubocovitch ML (eds) Advances in the biosciences, vol 18, Presynaptic receptors. Pergamon Press, New York, pp 321–325
Göthert M, Hentrich F (1984) Role of CAMP for regulation of impulse-evoked noradrenaline release from the rabbit pulmonary artery and its possible relationship to presynaptic ACTH receptors. Naunyn-Schmiedeberg's Arch Pharmacol 328:127–134
Göthert M, Hentrich F (1985) Identification of presynaptic β2-adrenoceptors on the sympathetic nerve fibres of the human pulmonary artery. Br J Pharmacol 85:933–941
Göthert M, Kollecker P (1986) Subendothelial β2-adrenoceptors in the rat vena cava: facilitation of noradrenaline release via local stimulation of angiotensin II synthesis. Naunyn-Schmiedeberg's Arch Pharmacol 334:156–165
Guimarães S, Brandão F, Paiva MQ (1978) A study of the adrenoceptor-mediated feedback mechanism by using adrenaline as a false transmitter. Naunyn-Schmiedeberg's Arch Pharmacol 305:185–188
Hentrich F, Göthert M, Greschushna D (1985) Involvement of cAMP in modulation of noradrenaline release in the human pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 330:245–247
Hope W, Law M, McCulloch MW, Rand MJ, Story DF (1976) Effects of some catecholamines on noradrenergic transmission in the rabbit ear artery. Clin Exp Pharmacol Physiol 3:15–28
Johnston H, Majewski H (1986) Prejunctional β-drenoceptors in the rabbit pulmonary artery and mouse atria: effect of α-adrenoceptor blockade and phosphodiesterase inhibition. Br J Pharmacol 87:553–562
Kawasaki H, Cline WE, Su C (1982) Enhanced beta-adrenoceptor-mediated modulation of vascular adrenergic neurotransmission in spontaneously hypertensive rats. J Pharmacol Exp Ther 223:721–728
Kubo T, Kuwahara M, Misu Y (1984) Effect of isoproterenol on vascular adrenergic neurotransmission in prehypertensive and hypertensive spontaneously hypertensive rats. Jpn J Pharmacol 36:419–421
Langer SZ, Enero MA, Adler-Graschinsky E, Dubocovitch ML, Celuch SM (1975) Presynaptic regulatory mechanisms for noradrenaline release by nerve stimulation. In: Davies DS, Reid JL (eds) Central action of drugs in blood pressure regulation. Pitman Medical, Tunbridge Wells, pp 133–150
Limberger N, Späth L, Starke K (1988) Presynaptic α2-adrenoceptor, opioid K-receptor and adenosine A1-receptor interactions on noradrenaline release in rabbit brain cortex. Naunyn-Schmiedeberg's Arch Pharmacol 338:53–61
Majewski H (1983) Modulation of noradrenaline release through activation of presynaptic β-adrenoceptors. J Anton Pharmacol 3:47–60. Corrigenda, p 155
Majewski H, Rand MJ (1981) An interaction between prejunctional α-adrenoceptors and prejunctional β-adrenoceptors. Eur J Pharmacol 69:493–498
Misu Y, Kubo T (1986) Presynaptic β-adrenoceptors. Med Res Rev 6:197–225
Misu Y, Kaiho M, Ogawa K, Kubo T (1981) Adrenergic transmission failure via the blockade of presynaptic beta receptors in guinea pig pulmonary arteries. J Pharmacol Exp Ther 218:242–247
Misu Y, Kuwahara M, Kaiho M, Kubo T (1983) Further characterization of presynaptic β-adrenoceptors in guinea-pig pulmonary arteries. Eur J Pharmacol 91:287–290
Misu Y, Kaiho M, Yasuda G, Kuwahara M, Kubo T (1984) Pharmacological properties of presynaptic β-adrenoceptors in guinea-pig pulmonary arteries. Jpn J Pharmacol 36:329–337
Molderings GJ, Likungu J, Zerkowski HR, Göthert M (1988) Presynaptic β2-adrenoceptors on the sympathetic nerve fibres of the human saphenous vein: no evidence for involvement in adrenaline-mediated positive feedback loop regulating noradrenergic transmission. Naunyn-Schmiedeberg's Arch Pharmacol 337:408–414
Nedergaard OA (1980) Modulation by the muscarinic agonist McNA-343 of noradrenaline release from vascular sympathetic neurones. J Cardiovasc Pharmacol 2:629–643
Nedergaard OA (1987) Effect of isoprenaline on noradrenaline release from sympathetic neurones in rabbit isolated pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 336:176–182
Nedergaard OA, Abrahamsen J (1990) Modulation of noradrenaline release by activation of presynaptic β-adrenoceptors in the cardiovascular system. Ann NY Acad Sci 604:528–544
Saelens DA, Williams PB (1983) Evidence for prejunctional α- and β-adrenoceptors in the canine saphenous vein: influence of frequency of stimulation and external calcium concentration. J Cardiovasc Pharmacol 5:598–603
Seamon KB, Daly JW (1986) Forskolin: its biological and chemical properties. Adv Cycl Nucleot Prot Phos Res 20:1–150
Starke K, Endo T, Taube HD (1975) Relative pre- and postsynaptic potencies of α-adrenoceptor agonists in the rabbit pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 291:55–78
Stevens MJ, Rittinghausen RE, Medcalf RL, Moulds RFW (1982) Prejunctional β-adrenoceptors in human digital arteries.Eur J Pharmacol 83:263–270
Stjärne L, Brundin J (1975)Dual adrenoceptor-mediated control of noradrenaline secretion from human vasoconstrictor nerves: facilitation by β-receptors and inhibition by α-receptors. Acta Physiol Scand 95:139–141
Stjarne L, Brundin J (1976) β2-Adrenoceptors facilitating noradrenaline secretion from human vasoconstrictor nerves. Acta Physiol Scand 97:88–93
Verbeuren TJ, Lorenz RR, Aarhus LL, Shepheard JT, Vanhoutte PM (1983) Prejunctional beta-adrenoceptors in human and canine saphenous veins. J Ant New Syst 8:261–271
Westfall TC, Peach MJ, Tittermary V (1979) Enhancement of the electrically induced release of norepinephrine from the rat portal vein: mediation by β2-adrenoceptors. Eur J Pharmacol 58:67–74
Westfall TC, Meldrum MJ, Badino L, Earnhardt JT (1984) Noradrenergic transmission in the isolated portal vein of the spontaneously hypertensive rat. Hypertension 6:267–274
Author information
Authors and Affiliations
Additional information
Send offprint requests to J. Abrahamsen at the above address
Rights and permissions
About this article
Cite this article
Abrahamsen, J., Nedergaard, O.A. Lack of presynaptic modulation by isoprenaline of 3H-noradrenaline release from rabbit isolated ear artery. Naunyn-Schmiedeberg's Arch Pharmacol 343, 161–165 (1991). https://doi.org/10.1007/BF00168604
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00168604