Summary
Isolated rabbit hearts were perfused at 34° (control), 24° or 12°C. The neuronal efflux of noradrenaline after perfusion with the amine for 1 h was depressed at 24° C (Q 10 about 5) in the presence or absence of desipramine; at 12°C the efflux was below the limit of estimation. Moderate reduction of the temperature (24° C) decreased the removal of perfused noradrenaline to about 60% of the control value and caused a 1.7-fold increase of the output of noradrenaline evoked by sympathetic nerve stimulation. It is concluded that the extremely temperature-dependent efflux of noradrenaline across the axonal membrane is not part of the release of noradrenaline evoked by nerve stimulation.
This is a preview of subscription content, log in via an institution to check access.
References
Euler, U. S. von, Lishajko, F.: Catecholamine release and uptake in isolated adrenergic nerve granules. Acta physiol. scand. 57, 468–480 (1963).
Graefe, K. H., Bönisch, H., Trendelenburg, U.: Time-dependent changes in neuronal net uptake of noradrenaline after pretreatment with pargyline and/or reserpine. Naunyn-Schmiedebergs Arch. Pharmak. 271, 1–28 (1971).
Green, R. D., Miller, J. W.: Evidence for the active transport of epinephrine and norepinephrine by the uterus of the rat. J. Pharmacol. exp. Ther. 152, 42–50 (1966).
Huković, S., Muscholl, E.: Die Noradrenalin-Abgabe aus dem isolierten Kaninchenherzen bei sympathischer Nervenreizung und ihre pharmakologische Beeinflussung. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 244, 81–96 (1962).
Iversen, L. L.: The uptake and storage of noradrenaline in sympathetic nerves. Cambridge: University Press 1967.
Kirpekar, S. M., Wakade, A. R.: Factors influencing noradrenaline uptake by the perfused spleen of the cat. J. Physiol. (Lond.) 194, 609–626 (1968).
Lindmar, R., Muscholl, E.: Die Wirkung von Pharmaka auf die Elimination von Noradrenalin aus der Perfusionsflüssigkeit und die Noradrenalinaufnahme in das isolierte Herz. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 247, 469–492 (1964).
Löffelholz, K.: Neuronal efflux of exogenous catecholamines from the isolated rabbit heart. Naunyn-Schmiedebergs Arch. Pharmacol. (in press) (1972).
Löffelholz, K.: Lindmar, R., Muscholl, E.: Analysis of wash-out of noradrenaline from the perfused rabbit heart after infusion of noradrenaline. Naunyn-Schmiedebergs Arch. Pharmak. 270, Suppl. R 87 (1971).
——, Muscholl, E.: Der Einfluß von d-Amphetamin auf die Noradrenalinabgabe aus dem isolierten Kaninchenherzen. Naunyn-Schmiedebergs Arch. Pharmak. 266, 393 (1970).
Paintal, A. S.: A comparison of the nerve impulses of mammalian nonmedullated nerve fibres with those of the smallest diameter medullated fibres. J. Physiol. (Lond.) 193, 523–533 (1967).
Smith, A. D., Winkler, H.: Fundamental mechanisms in the release of catecholamines. In: Handbook of experimental Pharmacology. H. Blaschko and E. Muschol, eds. Berlin-Heidelberg-New York: Springer 1972.
Trendelenburg, U., Draskócy, P. R.: The effect of intraneuronal inactivation on the transmembranal uptake of l- and d-norepinephrine. J. Pharmacol. exp. Ther. 175, 521–524 (1970).
Wennmalm, A., Hedquist, P., Stjärne, L.: Effects of hypothermia on norepinephrine release and effector response in isolated perfused cat spleen. Circulat. Res. 27, 39–44 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lindmar, R., Löffelholz, K. Differential effects of hypothermia on neuronal efflux, release and uptake of noradrenaline. Naunyn-Schmiedeberg's Arch. Pharmacol. 274, 410–414 (1972). https://doi.org/10.1007/BF00501278
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00501278