Abstract
In the nervous system of adult Schistosoma mansoni, neuropeptides and serotonin were demonstrated immunocytochemically. Neurons and nerve fibers immunoreactive to anti-FMRF-amide, anti-substance P, anti-leu-enkephalin, and anti-growth hormone releasing factor were found in the central and peripheral nervous system. The peptidergic nerve cell bodies in the bilobed brain are large and often multipolar; those in the peripheral nerve net are smaller and often bipolar. Sensory receptors of two morphologically different types were found along the surface of the worm. They are mainly immunoreactive to anti-substance P and anti-leu-enkephalin, and, to a lesser degree, to anti-FMRF-amide. Serotonin-immunoreactive fibers are found in the central and peripheral nervous elements as well as in sensory endings.
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References
Basch PF (1986) Internal chemical communication within flatworms. J Chem Ecol 12:1679–1686
Bennett J, Bueding E (1971) Localization of biogenic amines in Schistosoma mansoni. Comp Biochem Physiol [A] 39:859–867
Bruckner DA, Voge M (1974) The nervous system of larval Schistosoma mansoni as revealed by acetylcholinesterase staining. J Parasitol 60:437–446
Chan-Palay V, Palay SL (1984) Coexistence of neuroactive substances in neurons. Wiley & Sons, New York
Chia-Tung Pan S (1980) The fine structure of the miracidium of Schistosoma mansoni. J Invertebr Pathol 36:307–372
Chou TC, Bennett J, Bueding E (1972) Occurrence and concentrations of biogenic amines in trematodes. J Parasitol 58:1098–1102
Fairweather I, Maule AG, Mitchell SH, Johnston CF, Halton DW (1987) Immunocytochemical demonstration of 5-hydroxytryptamine (serotonin) in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea). Parasitol Res 73:255–258
Falkmer S, El-Salhy M, Titlbach M (1984) Evolution of the neuroendocrine system in vertebrates. A review with particular reference to the phylogeny and postnatal maturation of the islet parenchyma. In: Falkmer S, Håkanson R, Sundler F (eds) Evolution and tumour pathology of the neuroendocrine system. Elsevier Science, Amsterdam New York Oxford, pp 59–87
Gianutsos G, Bennett JL (1977) The regional distribution of dopamine and norepinephrine in Schistosoma mansoni and Fasciola hepatica. Comp Biochem Physiol [C] 58:157–159
Grimmelikhuijzen CJP (1984) Peptides in the nervous system of coelenterates. In: Falkmer S, Håkanson R, Sundler F (eds) Evolution and tumour pathology of the neuroendocrine system. Elsevier Science, Amsterdam New York Oxford, pp 39–58
Gustafsson MKS (1984) Synapses in Diphyllobothrium dendriticum (Cestoda). An electron microscopical study. Ann Zool Fenn 21:167–175
Gustafsson MKS (1985) Cestode neurotransmitters. Parasitol Today 1:72–75
Gustafsson MKS, Wikgren MC, Karhi TJ, Schot LPC (1985) Immunocytochemical demonstration of neuropeptides and serotonin in the tapeworm Diphyllobothrium dendriticum. Cell Tissue Res 240:255–260
Gustafsson MKS, Lehtonen MAI, Sundler F (1986) Immunocytochemical evidence for the presence of “mammalian” neurohormonal peptides in neurones of the tapeworm Diphyllobothrium dendriticum. Cell Tissue Res 243:41–49
Hockley DJ (1973) Ultrastructure of the tegument of Schistosoma. Adv Parasitol 11:233–305
Pax RA, Siefker C, Bennett JL (1984) Schistosoma mansoni: differences in acetylcholine, dopamine, and serotonin control of circular and longitudinal parasite muscles. Exp Parasitol 58:314–324
Reuter M (1987) Immunocytochemical demonstration of serotonin and neuropeptides in the nervous system of Gyrodactylus salaris (Monogenea). Acta Zool (Stockholm) 68:187–193
Reuter M, Karhi T, Schot LPC (1984) Immunocytochemical demonstration of peptidergic neurons in the central and peripheral nervous system of the flatworm Microstomum lineare with antiserum to FMRF-amide. Cell Tissue Res 238:431–436
Reuter M, Lehtonen M, Wikgren M (1987) Immunocytochemical evidence of neuroactive substances in flatworms of different taxa — a comparison. Acta Zool (Stockholm) 68:
Smyth JD, Halton DW (1983) The physiology of trematodes. Cambridge University Press, Cambridge
Sternberger LA (1974) Immunocytochemistry. In: Oster A, Weiss L (eds) Foundation of immunology series. Prentice Hall, Englewood Cliffs, NJ
Van Noorden S (1984) The neuroendocrine system in protostomian and deuterostomian invertebrates and lower vertebrates. In: Falkmer S, Håkanson R, Sundler F (eds) Evolution and tumour pathology of the neuroendocrine system. Elsevier Science, Amsterdam New York Oxford, pp 7–38
Wikgren M (1987) The neuroendocrine system of flatworms. Histochemical, immunocytochemical and ultrastructural studies of the microturbellarians Microstomum lineare and Stenostomum leucops and the cestode Diphyllobothrium dendriticum. PhD Thesis, åbo Akademi, Finland
Wikgren M, Reuter M (1985) Neuropeptides in a microturbellarian — whole mount immunocytochemistry. Peptides 6 [Suppl 3]:471–475
Wikgren M, Reuter M, Gustafsson M (1986) Neuropeptides in free-living and parasitic flatworms. An immunocytochemical study. Hydrobiologia 132:93–99
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Gustafsson, M.K.S. Immunocytochemical demonstration of neuropeptides and serotonin in the nervous system of adult Schistosoma mansoni . Parasitol Res 74, 168–174 (1987). https://doi.org/10.1007/BF00536029
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DOI: https://doi.org/10.1007/BF00536029