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Β-Adrenoceptor density on mononuclear leukocytes and right atrial myocardium in infants and children with congenital heart disease

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Summary

Sympathetic regulation of myocardial performance has been shown to be altered in congestive heart failure. Right atrial tissue of children with severe acyanotic and cyanotic congenital heart disease (CHD) showed a significantly lowerΒ-receptor density than that of children with less severe defects. Since mononuclear leukocytes (MNL) contain a homogeneous population ofΒ 2-adrenoceptors which have similar properties to those of cardiacΒ 2-adrenoceptors, they are frequently used for studying theΒ-adrenergic system. In a group of 37 children with CHD of different types and severity who underwent cardiac surgery, we compared the MNLΒ-adrenoceptor density to the type and severity of CHD and looked for a possible relationship to plasma catecholamine levels and to the right atrialΒ-adrenoceptor density. Membranes of MNL and myocardial cells were radiolabeled with (−)3-[125I]Iodocyanopindolol ([125I]ICYP). A significantly higherΒ-adrenoceptor density on MNL was found in patients with moderate acyanotic CHD (group I) than in those with severe acyanotic (group II) and cyanotic CHD (group III). Patients of group I showed approximatively 50% higher myocardialΒ-receptor density than those of groups II and III. ICI 118.551-[125I]ICYP competition studies revealed that in groups II and III significantly lower proportions and densities ofΒ 1-receptors were found compared to group I. Noradrenaline (NA) plasma levels in group II and group III were significantly higher than those in group I. The adrenaline plasma levels were found to be very high in all children with CHD. A significant negative correlation between NA levels and myocardial total andΒ 1-adrenoceptor density, but no correlation between plasma catecholamines and MNLΒ-adrenoceptor density, was calculated. We conclude that modulation of MNLΒ-adrenoceptors is not simply controlled by circulating catecholamine levels. CardiacΒ 2-adrenoceptor density remained unaltered, but theΒ 1-density was significantly lowered.Β 2-adrenoceptors on MNL showed a slight but significant decrease. However, cardiacΒ 2-adrenoceptor density cannot be predicted by measuring theΒ-adrenoceptor density on MNL.

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Abbreviations

CHD:

Congenital heart disease

MNL:

mononuclear leukocytes

NA:

noradrenaline

PPA/PAO :

Ratio between pulmonary and aortic pressure

Qp/Qs:

Ratio between pulmonary and systemic blood flow

HPLC-ED:

High performance liquid chromatography with electrochemical detector

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Authors and Affiliations

  1. Kinderpoliklinik der Ludwig-Maximilians-Universität, München

    R. Kozlik & D. Reinhardt

  2. Zentrum für Kinderheilkunde, Heinrich-Heine Universität, Düsseldorf

    H. H. Kramer & H. Wicht

  3. Abteilung für Thorax- und Kardiovaskuläre Chirurgie, Heinrich-Heine Universität, Düsseldorf

    W. Bircks

Authors
  1. R. Kozlik
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  2. H. H. Kramer
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  3. H. Wicht
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  4. W. Bircks
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  5. D. Reinhardt
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Kozlik, R., Kramer, H.H., Wicht, H. et al. Β-Adrenoceptor density on mononuclear leukocytes and right atrial myocardium in infants and children with congenital heart disease. Klin Wochenschr 69, 910–916 (1991). https://doi.org/10.1007/BF01798537

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  • DOI: https://doi.org/10.1007/BF01798537

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