Summary
An enzyme-linked immuno-absorbent assay has been developed for the detection of a circulating tumorassociated antigen, 90K, recognized by murine monoclonal antibody SP-2 (Iacobelliet al., Cancer Res 46: 3005–3010, 1986). This assay was found to be simple and reproducible. Using this method, 6% of 165 apparently healthy individuals and 15% of 91 patients with benign breast disease showed 90K levels above 1.7 units/ml. Approximately 50% of 129 patients with advanced breast cancer demonstrated serum antigen levels above 1.7 units/ml. All histological types of breast cancer were positive, and no association between the incidence of elevated 90K levels and other prognostic variables could be detected. The titers of 90K were significantly higher in sera from advanced-stage (3 and 4) patients than in those from patients with limited-stage (1 and 2) disease. Elevated 90K levels were also observed in patients with carcinomas of other sites, including gastrointestinal, gynecological, and lung tumors. By means of the immune blotting technique, the antigenic components carrying the determinants in serum and extracts of breast cancer cells have been identified. The levels of 90K did not correlate with those of CA 15-3 or CEA. The measurement of 90K in sera appears to be a useful adjunct to other available assays for the detection and monitoring of breast cancer and other malignant tumors.
References
Iacobelli S, Arno' E, D'Orazio A, Coletti G: Detection of antigens recognized by a novel monoclonal antibody in tissue and serum from patients with breast cancer. Cancer Res A6: 3005–3010, 1986
Avrameas S, Ternynch T: Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8: 1175–1179, 1971
Natoli C, Sica G, Natoli V, Serra A, Iacobelli S: Two new estrogen-supersensitive variants of MCF-7 human breast cancer cell line. Breast Cancer Res Treat 3: 23–32, 1983
Hearn M, Bethel G, Ayers J, Hancock W: Application of 1,1-carbonyldiimidazole-activated agarose for the purification of proteins, 2. The use of an activated matrix devoid of additional charged groups for the purification of thyroid proteins. J Chromatogr 185: 463–470, 1979
Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354, 1979
Harris JR, Hellman S, Canellos GP, Fisher B: Cancer of the breast.In De Vita VT, Hellman S, Rosenberg SA (eds); Cancer Principles and Practice of Oncology. J.B. Lippincott Company, Philadelphia, 1985, pp 1119–1177
Iacobelli S, Scambia G, Atlante G, Landoni F, Sismondi P, Vecchio FM: Effect of tamoxifen on steroid hormone receptors and creatine kinase activity in human endometrial carcinoma. Eur J Cancer Clin Oncol 22: 105–110, 1986
Snedecor GW, Cochran WG: Statistical Methods. The Iowa State University Press, Ames (Iowa), 1971
Colcher D, Hand P, Nuti M, Schlom J: A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc Natl Acad Sci USA 78: 3199–3203, 1981
Colcher D, Hand P, Wunderlick D, Nuti M, Teramato Y, Kufe D, Schlom J: Potential diagnostic and prognostic applications of monoclonal antibodies to human mammary carcinoma. In: Wright GL (ed) Monoclonal Antibodies and Cancer. Marcel Dekker, New York, 1983
Thompson CH, Jones SL, Whitehead RH, McKenzie IFC: A human breast tissue-associated antigen detected by a monoclonal antibody. J Natl Cancer Inst 70: 409–419, 1983
Coombes RC, Dearnaley DP, Ellison ML, Neville AM: Markers in breast and lung cancer. Ann Clin Biochem 19: 263–269, 1982
Waalkes TP, Enterline JP, Shaper JH, Abeloff MD, Ettinger DS: Biochemical markers for breast carcinoma. Cancer 53: 644–649, 1984
Edwards DP, Grzyb KT, Dressler LG, Mansel RE, Zava DT, Sledge GW, McGuire WL: Monoclonal antibody identification and characterization of a Mr 43,000 membrane glycoprotein associated with human breast cancer. Cancer Res 46: 1306–1317, 1986
Papsidero LD, Croghan GA, O'Connel MJ, Valenzuela LA, Nemoto T, Chu TM: Monoclonal antibodies (F36/22 and M7105) to human breast carcinoma. Cancer Res 43: 1741–1747, 1983
Burchell J, Wang D, Taylor-Papadimitriou J: Detection of the tumor-associated antigens recognized by the monoclonal antibodies HMFG-1 and 2 in serum from patients with breast cancer. Int J Cancer 34: 763–768, 1984
Hayes DF, Sekine H, Ohno T, Abe M, Keefe K, Kufe DW: Use of murine monoclonal antibody for detection of circulating DR3 plasma antigen levels in breast cancer patients. J Clin Invest 75: 1397–1402, 1985
Hilkens J, Kroenzen V, Bonfrer JMG, De Jong-Bakker M, Bruning PF: MAM-6 antigen, a new serum marker for breast cancer monitoring. Cancer Res 46: 2582–2587, 1986
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Iacobelli, S., Arno, E., Sismondi, P. et al. Measurement of a breast cancer associated antigen detected by monoclonal antibody SP-2 in sera of cancer patients. Breast Cancer Res Tr 11, 19–30 (1988). https://doi.org/10.1007/BF01807554
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DOI: https://doi.org/10.1007/BF01807554