Summary
Calcium is released from the isolated heavy sarcoplasmic reticulum (SR) of frog skeletal muscle upon application of 0.1–1 mM diethylpyrocarbonate (DEP, an imidazolyl reagent). The Ca-ATPase activity of SR was suppressed by 20% in the presence of 1 mM DEP. More than 1 mM of free magnesium ion or 5 μM ruthenium red eliminated the effect of DEP on calcium release but not on Ca-ATPase activity. A plausible site of DEP action is on the calcium channel.
Literature
Martonosi, A. N., Physiol. Rev.64 (1984) 1240.
Endo, M., Physiol. Rev.57 (1977) 71.
Winegrad, S., A. Rev. Physiol.44 (1982) 451.
Chiesi, M., and Inesi, G., J. biol. Chem.254 (1979) 10370.
Abramson, J. J., Trimm, J. L., Weden, L., and Salama, G., Proc. natl Acad. Sci. USA80 (1983) 1526.
Bindoli, A., and Fleischer, S., Archs Biochem. Biophys.221 (1983) 458.
Aoki, T., Oba, T., and Hotta, K., Can. J. Physiol. Pharmac.63 (1985) 1070.
Aoki, T., Oba, T., and Hotta, K., Can. J. Physiol. Pharmac.64 (1986) 1267.
Koshita, M., and Hotta, K., Jpn. J. Physiol.31 (1981) 109.
Nakamura, Y., Kobayashi, J., Gilmore, J., Mascal, M., Rinehart, K. L. Jr, Nakamura, H., and Ohizumi, Y., J. biol. Chem.261 (1986) 4193.
Fiske, C. H. and SubbaRow, Y., in: Methods in Enzymology III, p. 843. Eds S. P. Colowick and N. O. Kaplan. Academic press, New York 1957.
LeBel, D., Piorier, G. G., and Beaudoin, A. R., Analyt. Biochem.85 (1978) 86.
Ehrenberg, L., Fedorcsak, I., and Solymosy, F., Progr. nucl. Acid Res. molec. Biol.16 (1976) 189.
Fabiato, A., and Fabiato, F., J. Physiol. (Paris)75 (1979) 463.
Shoshan-Barmatz, V., Biochem. J.240 (1986) 509.
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Aoki, T., Oba, T. Calcium release from frog sarcoplasmic reticulum by an imidazolyl reagent. Experientia 45, 987–991 (1989). https://doi.org/10.1007/BF01953058
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DOI: https://doi.org/10.1007/BF01953058