Skip to main content
Log in

Adenosine constricts the isolated and perfused monkey coronary artery

  • Originals
  • Published:
Heart and Vessels Aims and scope Submit manuscript

Summary

Adenosine constricted 37 of 58 isolated and perfused monkey coronary arteries, whereas it dilated dog coronary arteries in all cases. The vasoconstriction induced by 0.1–10µg (0.37–37.4 nmol) of adenosine was examined, since adenosine in large doses produced tachyphylaxis. The adenosine-induced vasoconstriction was significantly attenuated by diltiazem (100µg [0.24µmol]), although it was not affected by aminophylline (100µg [0.23µmol]), phentolamine (100µg [0.36µmol]), or methysergide (10µg [28 nmol]. Forty-minute treatment with indomethacin (5 × 10−6 mol/l) completely prevented the adenosine-induced vasoconstriction, but it did not significantly inhibit vasoconstriction induced by KCl (3 mg [40µmol])- or norepinephrine (NE, 0.1µg [0.59 nmol]). These results suggest that: (1) adenosine constricts monkey coronary arteries by increasing Ca2+ influx across the smooth muscle cell membrane; (2) cyclooxygenase products may be involved in the adenosine-induced vasoconstriction; (3) adenosine receptors may not be involved in the vasoconstriction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Berne RM (1980) The role of adenosine in the regulation of coronary blood flow. Circ Res 47:807–813

    PubMed  Google Scholar 

  2. Arend LJ, Haramati A, Thompson CI, Spielman WS (1984) Adenosine-induced decrease in renin release: dissociation from hemodynamic effects. Am J Physiol 247:F447-F452

    PubMed  Google Scholar 

  3. Sakai K, Akima M (1978) Vasoconstriction after adenosine and inosine in the rat isolated hindlimb abolished by blockade of tryptaminergic mechanisms. Naunyn Schmiedebergs Arch Pharmacol 302:55–59

    PubMed  Google Scholar 

  4. Brown CM, Collis MG (1981) Adenosine contracts the isolated rat tail artery by releasing endogeneous 5-hydroxytryptamine. Eur J Pharmacol 76:275–277

    PubMed  Google Scholar 

  5. Nordstrom O, Delbro D (1986) Adenosine-induced contractions of the isolated rat trachea are mediated by 5-hydroxytryptamine. Acta Physiol Scand 127:557–558

    PubMed  Google Scholar 

  6. Biaggioni I, Enagat N, King L, Kubo K, Robertson D, Newman J (1987) Adenosine products pulmonary vasoconstriction through prostanoid mechanisms. Circulation (Suppl. VI):384

  7. Rossi N, Churchill P, Ellis V, Amore B (1988) Mechanism of adenosine receptor-induced renal vasoconstriction in rats. Am J Physiol 255:H885-H890

    PubMed  Google Scholar 

  8. Nakane T, Chiba S (1986) Comparison of vascular responses of isolated, perfused simian and canine coronary arteries to adrenergic agonists. Jpn Heart J 27:321–328

    PubMed  Google Scholar 

  9. Nakane T, Chiba S (1988) Constrictor responses of isolated and perfused monkey coronary arteries to pindolol. Jpn J Pharmacol 47:91–94

    PubMed  Google Scholar 

  10. Nakane T, Chiba S (1988) Comparative study of direct coronary vascular response to diltiazem, adrenergicβ-blockers and other vasodilators in isolated, perfused dog and monkey coronary arteries. Arch Int Pharmacodyn Ther 291:153–162

    PubMed  Google Scholar 

  11. Toda N (1983) Heterogeneity in the response to dopamine of monkey cerebral and peripheral arteries. Am J Physiol 245:H930-H936

    PubMed  Google Scholar 

  12. Tsuji T, Chiba S (1984) Potentiating effect of methysergide on norepinephrine-induced constriction of the internal carotid artery of the dog. Jpn J Pharmacol 34:95–100

    PubMed  Google Scholar 

  13. Rubanyi G, Vanhoutte PM (1985) Endothelium removal decreases relaxations of canine coronary arteries caused byβ-adrenergic agonists and adenosine. J Cardiovasc Pharmacol 7:139–144

    PubMed  Google Scholar 

  14. Rossi NF, Churchill PC, Jacobson KA, Leahy AE (1987) Further characterization of the renovascular effects of N6-cyclohexyladenosine in the isolated perfused rat kidney. J Pharmacol Exp Ther 240:911–915

    PubMed  Google Scholar 

  15. Moncada S, Vane JR (1979) Pharmacology and endogeneous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. Pharmacol Rev 30:293–331

    Google Scholar 

  16. Toda N (1984) Responses of human, monkey and dog coronary arteries in vitro to carbocyclic thromboxane A2 and vasodilators. Br J Pharmacol 83:399–408

    PubMed  Google Scholar 

  17. Shirahase H, Usui H, Manabe K, Kurahashi K, Fujiwara M (1988) Endothelium-dependent contraction and -independent relaxation induced by adenine nucleotides and nucleoside in the canine basilar artery. J Pharmacol Exp Ther 247:1152–1157

    PubMed  Google Scholar 

  18. Roberts AM, Messina EJ, Kaley G (1981) Prostaglandin (PGI2) mediated hypoxic relaxation of bovine coronary arterial strips. Prostaglandins 21:559–569

    Google Scholar 

  19. Palik I, Koltai MZ, Hadhäzy P, Molomvölgyi B, Wagner M, Pogätsa G (1982) Effects of prostaglandins E2, I2, and F on the tone of isolated coronary arteries from alloxan-diabetic dogs. Prostaglandins Leukotrienes Med 8:607–614

    Google Scholar 

  20. De May JG, Vanhoutte PM (1981) Role of the intima in cholinergic and purinergic relaxation of isolated canine femoral arteries. J Physiol 316:347–355

    PubMed  Google Scholar 

  21. White TD, Angus JA (1987) Relaxant effects of ATP and adenosine on canine large and small coronary arteries in vitro. Eur J Pharmacol 143:119–126

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nakane, T., Chiba, S. Adenosine constricts the isolated and perfused monkey coronary artery. Heart Vessels 5, 71–75 (1990). https://doi.org/10.1007/BF02058320

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02058320

Key words

Navigation