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Release of a humoral circulating cardioprotective factor by remote ischemic preconditioning is dependent on preserved neural pathways in diabetic patients

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Abstract

Efficacy of ischemic preconditioning is decreased in animal models of type 2 diabetes mellitus while the responses in humans with diabetes are contradictory. It is unknown whether attenuation is related to decreased release of a mediating humoral cardioprotective factor or reduced ability to respond in the target tissue. The aim of the present study was to investigate the release and effect of a circulating cardioprotective factor in type 2 diabetes mellitus patients. Blood samples were drawn from nine non-diabetic subjects, eight diabetic patients without peripheral neuropathy, and eight diabetic patients with peripheral neuropathy before (control) and after a remote ischemic preconditioning (rIPC) stimulus. Blood samples were dialyzed against Krebs–Henseleit buffer and the cardioprotective effects of the dialysates were tested in rabbit hearts mounted on a Langendorff model and subjected to 30-min global ischemia and 120-min reperfusion. rIPC dialysate from non-diabetic and diabetic subjects without peripheral neuropathy reduced infarct size and improved hemodynamic recovery compared to control dialysate from non-diabetic and diabetic subjects. However, in the subgroup of diabetic patients with neuropathy the cardioprotective effect was attenuated. These findings indicate that the release mechanism involves neural pathways.

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Acknowledgments

The study was funded by Leducq (06CVD), The Danish Research Council (11-108354), The Danish Strategic Research Council (11-115818), and Aarhus University Institute of Clinical Medicine. The skillful technical assistance of Eva Sparrewath and Bente Jacobsen is greatly appreciated.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Cardiology, Aarhus University Hospital, Skejby, Brendstrupgaardsvej 100, 8200, Aarhus N, Denmark

    Rebekka Vibjerg Jensen, Nicolaj Brejnholt Støttrup, Steen Buus Kristiansen & Hans Erik Bøtker

  2. Institute of Clinical Medicine, Aarhus University, Brendstrupgaardsvej 100, 8200, Aarhus N, Denmark

    Rebekka Vibjerg Jensen, Nicolaj Brejnholt Støttrup, Steen Buus Kristiansen & Hans Erik Bøtker

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  1. Rebekka Vibjerg Jensen
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Correspondence to Rebekka Vibjerg Jensen.

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Jensen, R.V., Støttrup, N.B., Kristiansen, S.B. et al. Release of a humoral circulating cardioprotective factor by remote ischemic preconditioning is dependent on preserved neural pathways in diabetic patients. Basic Res Cardiol 107, 285 (2012). https://doi.org/10.1007/s00395-012-0285-1

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  • DOI: https://doi.org/10.1007/s00395-012-0285-1

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