Abstract
The peptide angiotensin-(1–7) [Ang-(1–7)] is known to enhance water transport in rat inner medullary collecting duct (IMCD). The aim of this study was to determine the mechanism of the Ang-(1–7) effect on osmotic water permeability (P f). P f was measured in the normal rat IMCD perfused in vitro in presence of agonists [Ang-(1–7), arginine vasopressin (AVP) and Ang-(3–8)], and antagonists of the angiotensin and the vasopressin cascade. Ang-(1–7), but not Ang-(3–8), increased P f significantly. The effect of Ang-(1–7) on P f was abolished by its selective antagonist, A-779, added before or after Ang-(1–7). Prostaglandin E2 and the protein kinase A inhibitor H8 also blocked the Ang-(1–7) effect. Blockade of vasopressin V1 receptors by antagonists did not change the Ang-(1–7) effect, but pre-treatment with a V2 antagonist abolished the effect of Ang-(1–7) on P f. Similarly, pre-treatment with A-779 inhibited AVP’s effect on P f. Forskolin-stimulated P f was blocked both by A-779 and by the V2 antagonist. Finally, Ang-(1–7) increased cAMP levels in fresh IMCD cell suspensions whilst the forskolin-stimulated cAMP synthesis was decreased by A-779 and the V2 antagonist. These data provide evidence that Ang-(1–7) interacts via its receptor with the AVP V2 system through a mechanism involving adenylate-cyclase activation.
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Acknowledgements
This work was supported by PRONEX-CNPq, LIM HCFMUSP and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). The Laboratory LIM 12 is also supported by the Fundação Faculdade de Medicina (FFM). A J Magaldi and RAS Santos were supported in part by a fellowship from Conselho Nacional de Pesquisa-CNPq. Parts of this work were presented at the XIIIth Int Cong Nephrol Madrid p 53, 1995 and in the XXXIVth Cong Eur Renal Ass Genebra, p10, 1997.
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Magaldi, A.J., Cesar, K.R., de Araújo, M. et al. Angiotensin-(1–7) stimulates water transport in rat inner medullary collecting duct: evidence for involvement of vasopressin V2 receptors. Pflugers Arch - Eur J Physiol 447, 223–230 (2003). https://doi.org/10.1007/s00424-003-1173-1
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DOI: https://doi.org/10.1007/s00424-003-1173-1