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Distinct Expression of Mas1-Related G-Protein-Coupled Receptor B4 in Dorsal Root and Trigeminal Ganglia—Implications for Altered Behaviors in Acid-Sensing Ion Channel 3-Deficient Mice

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Abstract

Mas1-related G-protein-coupled receptors (Mrgprs), comprising more than 50 distinct members, are specifically expressed in primary sensory neurons. Reflecting the diversity and specificity of stimuli they detect, Mrgprs are involved in pain, touch, and itch-related behaviors. Sensory–neuron-specific acid-sensing ion channel 3 (ASIC3) is essential for touch and inflammatory pain, but mice lacking ASIC3 have complex behavioral alterations in various modalities of pain and touch. To understand whether Mrgprs are involved in complex behavioral alterations found in ASIC3-deficient mice, we examined Mrgpr gene expression in ASIC3−/− mice. Only MrgprB4 expression has shown significant change. MrgprB4 expression was increased in ASIC3−/− dorsal root ganglia (DRG) but decreased in ASIC3−/− trigeminal ganglia. The distinct alterations in DRG and trigeminal ganglia imply that MrgprB4 could have multiple functions. Given that MrgprB4 is expressed in neurons that may detect gentle touch and that ASIC3−/− mice have altered sensitivity of mechanoreceptors for light touch, the expression change of MrgprB4 is more likely related to the altered touch behaviors of ASIC3−/− mice.

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Acknowledgments

We thank the support of the Institute of Neuroscience at National Yang Ming University for the calcium imaging system. This work was supported by funds from National Science Council, Taiwan (grant no. NSC101-2321-B-008-001), and from National Central University and Landseed Hospital, Taiwan (grant no. NCU-LSH-101-A-002).

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  1. Department of Life Sciences, National Central University, Jhongda Road 300, 32054, Jhongli, Taiwan

    Ya-Han Huang, Chin-Yu Chang & Wei-Hsin Sun

  2. Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan

    Chih-Cheng Chen

  3. Landseed Hospital, Jhongli, Taiwan

    Chih-Dong Yang

  4. Institute of Systems Biology and Bioinformatics, National Central University, Jhongli, Taiwan

    Wei-Hsin Sun

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  1. Ya-Han Huang
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Correspondence to Wei-Hsin Sun.

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Huang, YH., Chang, CY., Chen, CC. et al. Distinct Expression of Mas1-Related G-Protein-Coupled Receptor B4 in Dorsal Root and Trigeminal Ganglia—Implications for Altered Behaviors in Acid-Sensing Ion Channel 3-Deficient Mice. J Mol Neurosci 51, 820–834 (2013). https://doi.org/10.1007/s12031-013-0070-0

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