Isolation of a full-length cDNA encoding mouse aromatase P450☆,☆☆

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Abstract

A full-length cDNA clone for aromatase P450 has been isolated from a pregnant mouse ovarian cDNA library. The insert of this clone (2394 bp) contains a 1509-bp open reading frame encoding 503 amino acid residues together with a 46-bp 5′-untranslated stretch and an 839-bp 3′-untranslated region to which a poly(A) tract is attached. Northern blot analysis of ovarian RNA from pregnant mice reveals a major mRNA band of 2.5 kb with a minor band of 2.1 kb. Comparison of mouse aromatase P450 with that of rat, human, and chicken shows 91, 81, and 69% identity in the nucleotide sequence and 92, 79, and 69% identity in the deduced amino acid sequence, respectively. The membrane-spanning domain of mouse aromatase P450 is estimated to be an extremely hydrophobic segment located within the N-terminal region of the molecule. Furthermore, a highly conserved heme-binding domain is noticed.

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    ☆

    This work was supported in part by research grants from Toray Science Foundation, Special Education and Research Fund of Kochi Medical School, and by Grants-in-Aid for Science and Cancer Research from the Ministry of Education, Science, and Culture of Japan.

    ☆☆

    The nucleotide sequence of the mouse cDNA reported in this paper will appear in the GenBank, EMBL, and DDBJ Nucleotide Sequence Database under Accession Number D00659.

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