Elsevier

Biochemical Pharmacology

Volume 24, Issue 22, 15 November 1975, Pages 2089-2095
Biochemical Pharmacology

Effects of aldehydes on sodium plus potassium ion-stimulated adenosine triphosphatase of mouse brain

https://doi.org/10.1016/0006-2952(75)90107-0Get rights and content

Abstract

The biogenic aldehydes, 3,4-dihydroxyphenylglycolaldehyde and 3,4-dihydroxyphenylacetal-dehyde, derived from norepinephrine and dopamine, respectively, as well as acetaldehyde, porpionalde-hyde, benzaldehyde and phenylacetaldehyde, inhibited both Na+ + K+-activated ATPase and Mg2+-ATPase. In addition, K+ ion-dependent p-nitrophenylphosphatase activity was inhibited by these compounds. The Na+ + K+-ATPase was much more sensitive than Mg2+-ATPase of K+-activated phosphatase to inhibition by various aldehydes. The inhibition of Na+ + K+-ATPase by aldehydes was reversible and was non-competitive with ATP or K+ as the variable substrate or activator respectively. Addition of cysteine or mercaptoethanol protected the enzymes from inhibition by aldehydes. The concentrations of aldehydes which produced marked inhibition of Na+ + K+-ATPase ranged from 2 × 10−2 M to 6 × 10−6 M for acetaldehyde and 3,4-dihydroxyphenylglycoladehyde respectively. All aldehydes, including acetaldehyde, were more potent inhibitors of Na+ + K+-ATPase activity than was ethanol.

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    This work was supported in part by Public Health Service Grants MH 18948 and AA 00293, and in part by a grant from the Wyoming Heart Association.

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