Use of isolated kidney cells for study of drug metabolism
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Cited by (136)
Resveratrol, curcumin and gallic acid attenuate glyoxal-induced damage to rat renal cells
2020, Toxicology ReportsCitation Excerpt :Furthermore, the low antioxidant capacity of the cell intensifies the damage caused by radicals leading to the eventual cell death. Also, glucose is changed to sorbitol when subjected to hyperglycemic conditions, leading to the overproduction of superoxide anion radicals and reduction of intracellular concentrations of agents that are involved in the cell antioxidant defense (e.g. NADPH and GSH), as well as increment of the risk of oxidative stress occurrence [47,83]. It should be mentioned that biological membranes are highly susceptible to damage caused by ROS and such damage increases lipid peroxidation if not inhibited by the cellular antioxidant defense mechanism(s) [83].
The role of biotransformation and oxidative stress in 3,5-dichloroaniline (3,5-DCA) induced nephrotoxicity in isolated renal cortical cells from male Fischer 344 rats
2016, ToxicologyCitation Excerpt :All chemicals used were the highest purity available and were purchased from Sigma–Aldrich (St. Louis, MO) or Fisher Scientific (Pittsburgh, PA). Untreated male rats were anesthetized with pentobarbital (75 mg/kg, ip) and isolated renal cortical cells (IRCC) were obtained using the collagenase perfusion method of Jones et al. (1979). Cell viability was initially determined by trypan blue (2% w/v) exclusion and lactate dehydrogenase (LDH) release.
4-Amino-2-chlorophenol: Comparative in vitro nephrotoxicity and mechanisms of bioactivation
2014, Chemico-Biological InteractionsCitation Excerpt :For each compound, the highest purity level available was used. Untreated rats were anesthetized (pentobarbital sodium, 75 mg/kg, ip) and isolated rat renal cortical cells (IRCC) were obtained via the collagenase perfusion method of Jones et al. [28]. Initial cell viability was typically 85–95% as determined by trypan blue (2% w/v) exclusion and initial lactate dehydrogenase (LDH) release (∼5–10%).
Modulation of mitochondrial glutathione status and cellular energetics in primary cultures of proximal tubular cells from remnant kidney of uninephrectomized rats
2013, Biochemical PharmacologyCitation Excerpt :Control and NPX rats were age-matched for all studies. Isolated renal cortical cells were obtained by collagenase perfusion [30]. PT cells were enriched from the cortical cells by Percoll density-gradient centrifugation [31] and the PT cells were then placed into primary culture [32].
Diabetes increases susceptibility of primary cultures of rat proximal tubular cells to chemically induced injury
2009, Toxicology and Applied Pharmacology