Elsevier

Biochemical Pharmacology

Volume 33, Issue 20, 15 October 1984, Pages 3241-3245
Biochemical Pharmacology

A novel biologically active seleno-organic compound—II: Activity of PZ 51 in relation to Glutathione Peroxidase

https://doi.org/10.1016/0006-2952(84)90084-4Get rights and content

Abstract

The anti-inflammatory compound 2-phenyl-1,2-benzoisoselenazol-3(2H)-on (PZ 51) catalysed GSSG formation from GSH in the presence of hydroperoxides in an NADPH/GSSG reductase system with the following rates (Δ log GSH/min per molar selenium): 1.1 × 106 with H2O2, 1.2 × 106 with butylhydroperoxide, 1.7 × 106 with cumenehydroperoxide. The reaction catalysed by the sulphur analogue of PZ 51 was negligible. Similar results were obtained in a direct assay of GSH-Px activity based on GSH estimation by dithionitrobenzoate. The activation energy of the reaction was determined as 55 kJ/mol · deg in the presence of 30 μmol/l PZ 51 compared to 36.5 kJ/mol · deg obtained in the presence of 1 nmol/l pure GSH-Px isolated from bovine red blood cells. In mouse liver microsomes, NADPH-dependent aminopyrine dealkylation was totally inhibited in the presence of 50 μmol/l PZ 51. In vivo experiments with Se-deficient mice showed that the Se-moiety of PZ 51 is not available for the synthesis of the selenoenzyme GSH-Px after dietary treatment or i.p. doses up to 25 mg Se as PZ 51 per kg body wt. After oral administration of labelled PZ 51, unlike with selenite, no radioactivity was incorporated into GSH-Px within 48 hr. The data suggest that several similarities between PZ 51 and the active site of GSH-Px exist, resulting in the capability of the compound to catalyse the GSH-Px reaction. An extracellular pharmacodynamic action of the drug seems likely.

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