Elsevier

Biochemical Pharmacology

Volume 51, Issue 9, 3 May 1996, Pages 1117-1122
Biochemical Pharmacology

Research paper
Gender-differential liver plasma membrane affinities in hepatic tetrabromosulfonephthalein (TBS) uptake

https://doi.org/10.1016/0006-2952(95)02407-7Get rights and content

Abstract

The sex difference in the hepatic uptake of tetrabromosulfonephthalein (TBS) was investigated in male and female rats in two different experimental models. In the intact animal, the initial plasma disappearance constant rate, the initial velocity of uptake, and the plasma clearance of TBS were significantly higher in females than in males. In sinusoidal liver plasma membrane vesicles, kinetic parameters of TBS uptake were investigated in both sexes. The Km was lower in females than in males (5.5 ± 0.4 vs 17 ± 4 μM, P < 0.05), whereas Vmax showed comparable values (544 ± 15 vs 581 ± 60 nmol TBS/min/mg protein, mean ± SD, NS, females and males, respectively). Collectively, these data indicate that the sex difference in hepatic uptake of TBS is located at the sinusoidal liver plasma membrane and is due to a greater affinity of the electrogenic transport system(s) in females.

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This work was supported by the following awards: Fundacidn Antorchas (Subsidio para la Reins&&n de Becurios Extemos 1993)) Third World Academy of Sciences (TWAS Research Grant 93-422 RG/BIO/LA), and Universidad National de Rosario (PID 003-202/ 93-02).

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