The maintenance and identification of mouse cerebellar granule cells in monolayer culture
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Cellular models of Batten disease
2020, Biochimica et Biophysica Acta - Molecular Basis of DiseaseCitation Excerpt :Cerebellar granule cells (CGCs) have long been recognised as being a ubiquitously occurring within the mammalian brain [94]. First isolated from murine models for cell culture in the 1970s, the dissociation and growth of cerebellar granule cells have expedited the understanding of neuronal functionality in both wild-type and a disease-based context [95]. Several murine models recapitulating multiple CLN mutations have been produced from labs worldwide.
A low-density culture method of cerebellar granule neurons with paracrine support applicable for the study of neuronal morphogenesis
2013, Brain ResearchCitation Excerpt :In this culture, hippocampal neurons extend short immature neurites, which become polarized by establishing axonal and dendritic compartments (Dotti et al., 1988; Wiggin et al., 2005; Arimura and Kaibuchi, 2007). This system has therefore enabled single cell analysis of neuronal morphogenesis, and consequently the discovery of molecular mechanisms involved in neuronal polarity (Shi et al., 2003; Jacobson et al., 2006; Shelly et al., 2007; Konishi and Setou, 2009). However, a key issue that remains is whether the polarity factors found in this paradigm are also involved in the formation of axonal and dendritic compartments of other neurons.
Preventing H<inf>2</inf>O<inf>2</inf>-induced apoptosis in cerebellar granule neurons by regulating the VEGFR-2/Akt signaling pathway using a novel dimeric antiacetylcholinesterase bis(12)-hupyridone
2011, Brain ResearchCitation Excerpt :Cytosine arabinoside (10 μM) was added to the culture medium 24 h after plating to limit the growth of non-neuronal cells. Granule cells were identified by a combination of several criteria including their size, shape and relative proportion of the total cell population as determined by phase contrast microscopy (Messer, 1977). With the use of this protocol, more than 95% of the cultured cells were granule neurons.
Long-term kainic acid exposure reveals compartmentation of glutamate and glutamine metabolism in cultured cerebellar neurons
2007, Neurochemistry International