Elsevier

Brain Research

Volume 461, Issue 2, 4 October 1988, Pages 230-237
Brain Research

Single or repeated mild stress increases synthesis and release of hypothalamic corticotropin-releasing factor

https://doi.org/10.1016/0006-8993(88)90254-5Get rights and content

Abstract

The effect of a specific mild stress on the levels of corticotropin-releasing factor immunoreactivity (CRF-ir) in the hypothalamus of adult male rats was determined using a radioimmunoassay specific for rat CRF. A single 5 min restraint significantly increased CRF-ir in the median eminence 24 h later compared to appropriate controls (P < 0.025), with no change detected earlier. Plasma ACTH, an indirect index of CRF release, was significantly elevated within 15 min (P < 0.0025). Repetition of mild stress daily for 9 days (P < 0.01), or a single episode of handling (P < 0.05), both resulted in significantly increased CRF-ir in the whole hypothalamus 24 h later. Blockade of axonal transport by intracisternal colchicine decreased CRF-ir in the median eminence 24 h later (P < 0.005). Inhibition of protein synthesis by anisomycin during a single 5 minute restraint resulted in significantly decreased CRF-ir in the median eminence 24 h later compared to vehicle-injected stressed rats (P < 0.005) or to anisomycin-injected unstressed controls (P < 0.025). These data show that mild stress increased net hypothalamic CRF content as a result of the balance between augmented synthesis and augmented release.

References (47)

  • KantG.J. et al.

    Comparison of the effects of CRF and stress on levels of pituitary cyclic AMP and plasma ACTH in vivo

    Peptides

    (1986)
  • MasonJ.W.

    A re-evaluation of the concept of ‘non-specificity’ in stress theory

    J. Psychiatry Res.

    (1971)
  • MoldowR.L. et al.

    Stress mediated changes in hypothalamic corticotropin releasing factor-like immunoreactivity

    Life Sci.

    (1987)
  • ParéW.P. et al.

    Restraint stress in biomedical research: a review

    Neurosci. Biobehav. Rev.

    (1986)
  • QuirceC.M. et al.

    The effects of predictable and unpredictable schedules of physical restraint upon rats

    Life Sci.

    (1981)
  • RainbowT.C. et al.

    Anisomycin inhibits the activation of sexual behavior by estradiol and progesterone

    Brain Research

    (1980)
  • SkofitschG. et al.

    Distribution of corticotropin-releasing factor-like immunoreactivity in the rat brain by immunohistochemistry and radioimmunoassay

    Peptides

    (1985)
  • TorrellasA. et al.

    Adrenal hormones and brain catecholamines responses to morning and afternoon immobilization stress in rats

    Physiol. Behav.

    (1981)
  • BarrettA.M. et al.

    The effect of housing conditions and simple experimental procedures upon the corticosterone level in the plasma of rats

    J. Endocrinol.

    (1963)
  • BennettE.L. et al.

    Cerebral protein synthesis inhibition and amnesia produced by scopolamine, cycloheximide, streptovitacin A, anisomycin and emetine in rat

  • BrownM.R. et al.

    Corticotropin-releasing factor: effects on the autonomic nervous system and visceral systems

  • BruhnT.O. et al.

    Effect of paraventricular lesions on corticotropin-releasing factor (CRF)-like immunoreactivity in the stalk-median eminence: studies on the adrenocorticotropin response to ether stress and exogenous CRF

    Endocrinology

    (1984)
  • ChappellP.B. et al.

    Alterations in corticotropin-releasing factor-like immunoreactivity in discrete rat brain regions after acute and chronic stress

    J. Neurosci.

    (1986)
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