Elsevier

Brain Research

Volume 457, Issue 2, 9 August 1988, Pages 259-266
Brain Research

Inhibition of striatal acetylcholine release by endogenous serotonin

https://doi.org/10.1016/0006-8993(88)90694-4Get rights and content

Abstract

We have examined the hypothesis that endogenous serotonin (5-HT) exerts an inhibitory influence on the release of acetylcholine (ACh) in striatum. Striatal slices were prepared from adult rats, preincubated with [3H]choline, superfused, and exposed to electrical field stimulation. The stimulation-induced overflow of tritium into the superfusate was used as a measure of ACh release. We observed that fluoxetine, an inhibitor of 5-HT uptake, reduced ACh overflow in slices prepared from caudal striatum, an area of high 5-HT concentration, but not in slices from rostral striatum, an area of low 5-HT concentration. Moreover, methysergide, a 5-HT antagonist, increased ACh efflux in caudal but not rostral striatum. Finally, direct activation of 5-HT receptors with the 5-HT agonist, quipazine, inhibited stimulation-induced ACh overflow in both rostral and caudal striatum. These results suggest that endogenous 5-HT normally is capable of inhibiting striatal ACh release, and that the extent of the modulation is related to the degree of serotonergic innervation. In addition, 5-HT receptors capable of modulating ACh release are present in 5-HT-poor rostral striatum, as well as in 5-HT-rich caudal striatum.

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    The far more profuse other 5-HT terminal endings that we identified have also innervated the GABA projection neurons. These results suggest that 5-HT fibers terminate not only on the intrinsic ChAT cells but also on the GABA projection neurons, and that 5-HT fibers exert inhibitory [44–47] and/or modulatory effects [48] on the activities of both intrinsic and projection neurons depending on their receptor characteristics such as 5-HT1 or 5-HT2 [49–52]. It is well established that the striatum is innervated by 5-HT neurons.

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*

Present address: National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, U.S.A.

**

Present address: Department of Psychology, 48 Townshend Hall, Ohio State University, Columbus, OH 43210, U.S.A.

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