Elsevier

Brain Research

Volume 691, Issues 1–2, 11 September 1995, Pages 9-17
Brain Research

Research report
Effect of muscimol on haloperidol-induced alteration of neurotensin gene expression in the striatum and nucleus accumbens in the rat

https://doi.org/10.1016/0006-8993(95)00573-9Get rights and content

Abstract

Acute neuroleptic administration increases the expression of neurotensin/neuromedin (NT/N) gene in rat dorsolateral striatum and shall sector of the nucleus accumbens. The purpose of this study was to examine modulation of neuroleptic induction of NT/N and the proto-oncogene c-fos expression by the GABAA agonist muscimol. Adult male Sprague-Dawley rats were treated with saline, haloperidol (1 mg/kg); muscimol (3.2 mg/kg); or haloperidol (1 mg/kg) plus muscimol (3.2 mg/kg). Animals were sacrificed 1 h after drug administration. Expression of NT/N and c-fos mRNA was examined by in situ hybridization using 35S-antisense probes. Muscimol alone had no measurable effect on basal levels of NT/N or c-fos mRNA in either the dorsolateral striatum or the nucleus accumbens. However, co-administration of muscimol with haloperidol reduced haloperidol-induced increases in NT/N as well as c-fos mRNA in the dorsolateral striatum. In contrast, NT/N mRNA expression in accumbal shell induced by haloperidol was not modulated by co-administration of muscimol. These data suggest that GABAA receptors may be involved in regulation of NT/N gene expression in the DLSt, but not in the nucleus accumbens.

References (43)

  • K.M. Merchant et al.

    Co-expression of neurotensin and c-fos mRNAs in rat neostriatal neurons following acute haloperidol

    Mol. Brain Res.

    (1994)
  • K.M. Merchant et al.

    Haloperidol rapidly increases the number of neurotensin mRNA-expressing neurons in neostriatum of the rat brain

    Brain Res.

    (1991)
  • W.T. O'Connor et al.

    The effects of neurotensin on GABA and acetylcholine release in the dorsal striatum of the rat: An in vivo microdialysis study

    Brain Res.

    (1992)
  • S.O. Ogren et al.

    D1-and D2-receptor antagonists induce catalepsy via different efferent striatal pathways

    Neurosci. Lett.

    (1988)
  • Z.N. Stowe et al.

    The electrophysiological actions of neurotensin in the central nervous system

    Life Sci.

    (1991)
  • G.R. Uhl et al.

    Regional and subcellular distribution of brain neurotensin

    Life Sci.

    (1976)
  • W.S. Young et al.

    Neurotensin receptor localization by light microscopic autoradiography in rat brain

    Brain Res.

    (1981)
  • D.S. Zahm et al.

    On the significance of subterritories in the ‘accumbens’ part of the ventral striatum

    Neuroscience

    (1992)
  • U. Andersson et al.

    GABA agonists in cebus monkeys with neuroleptic-induced persistent dyskinesias

    Psychopharmacology

    (1988)
  • E. Costa et al.

    Action of antischizophrenic drugs on the metabolism of γ-aminobutyric acid and acetylcholine in globus pallidus, striatum and n. accumbens

  • R. Corbett et al.

    GABAminetic agents display anxiolytic-like effects in the social interaction and elevated plus maze procedures

    Psychopharmacology

    (1991)
  • Cited by (11)

    • Neurotransmitter Regulation of Basal Ganglia Gene Expression

      2010, Handbook of Behavioral Neuroscience
      Citation Excerpt :

      First, both the initial (Dragunow et al., 1990; Miller, 1990) and numerous subsequent studies have established that D2 receptor antagonists, by removing this inhibitory influence, increase gene expression in the dorsal striatum. Such D2 receptor antagonist-mediated increases in gene expression are evident for c-fos (Merchant and Dorsa, 1993; Ziólkowska and Höllt, 1993; Sirinathsinghji et al., 1994; Decker et al., 1995; MacGibbon et al., 1995; Boegman and Vincent, 1996; Keefe and Adams, 1998; de Souza and Meredith, 1999; Steiner and Gerfen, 1999; Adams and Keefe, 2000; Hussain et al., 2002; Pillot et al., 2002; Lee and Rajakumar, 2003; Binder et al., 2004; Ethier et al., 2004; Yanahashi et al., 2004; Robbins et al., 2008), zif268 (MacGibbon et al., 1995; Keefe and Adams, 1998; Steiner and Gerfen, 1999; Adams and Keefe, 2000), neurotensin/neuromedin N (Nts) (Merchant and Dorsa, 1993; Senger et al., 1993; Sirinathsinghji et al., 1994; Decker et al., 1995; Augood et al., 1997; Pillot et al., 2003), fosB (MacGibbon et al., 1995), junB (Sirinathsinghji et al., 1994; MacGibbon et al., 1995), junD (MacGibbon et al., 1995), krox20 (egr2) (MacGibbon et al., 1995; Robbins et al., 2008), ngfi-B (Nr4a1) (Beaudry et al., 2000), homer1/ania-3 (de Bartolomeis et al., 2002; Polese et al., 2002; Tomasetti et al., 2007), ppe (Pillot et al., 2003), and arc (Nakahara et al., 2000; Robbins et al., 2008; Fumagalli et al., 2009). Second, D2 receptor agonists can suppress gene expression (e.g., zif268, c-fos, ngfi-B, junB) in striatum (Gerfen et al., 1995; Svenningsson et al., 1995; Le Moine et al., 1997), suggesting that tonic dopamine signaling does not maximally inhibit basal expression.

    View all citing articles on Scopus
    View full text