Elsevier

Cellular Immunology

Volume 137, Issue 1, 1 October 1991, Pages 36-45
Cellular Immunology

Cyclic AMP-mediated modulation of immunoglobulin production in B cells by prostaglandin E1

https://doi.org/10.1016/0008-8749(91)90054-FGet rights and content

Abstract

We had previously demonstrated in a transformed human B cell line, LA350, the existence of an inverse relationship between cyclic adenosine monophosphate (cAMP) content and immunoglobulin secretion using the cAMP-elevating agents such as cholera toxin and forskolin. In this paper we report that cAMP acting as a second messenger for prostaglandin exerts a similar effect on the antibody response of B lymphocytes. Incubation of the cells with PGE1 in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) produced a concentration- and time-dependent elevation of intracellular cAMP. Significant increases of cAMP production were observed at physiologically relevant levels of PGE1 (10−7 and 10−8, M). Immunoglobulin production, whether measured as the total number of immunoglobulin-secreting cells by a reverse hemolytic plaque assay or as specific immunoglobulin production (IgM) by an enzyme-linked immunoadsorbent assay, was suppressed in a dose-dependent fashion by the presence of IBMX. This suppression of immunoglobulin production was significantly enhanced by the presence of PGE1. Phorbol myristate acetate-induced IgM production was also inhibited by the presence of PGE1. These results imply that prostaglandins regulate B cell activation and immunoglobulin production by signal transduction mechanisms involving cyclic nucleotides.

References (45)

  • W.S. Lapp et al.

    Cell Immunol

    (1980)
  • M.A. Bray et al.

    Prostaglandins Med

    (1978)
  • M. Lagarde et al.

    Prog. Lipid Res

    (1981)
  • S. Bauminger

    Prostaglandins

    (1978)
  • D.R. Webb et al.

    Cell. Immunol

    (1978)
  • J.L. Ceuppens et al.

    Cell. Immunol

    (1982)
  • J.S. Goodwin et al.

    Cell. Immunol

    (1979)
  • U. Erten et al.

    Prostaglandins Med

    (1980)
  • D. Mary et al.

    J. Biol. Chem

    (1989)
  • E.L. Morgan

    Clin. Immunol. Immunopathol

    (1987)
  • T. Morito et al.

    Prostaglandin

    (1980)
  • I.L. Bonta et al.

    Int. J. Immunopharmacol

    (1984)
  • C. Nordstedt et al.

    FEBS Lett

    (1987)
  • V. Dayal et al.

    Biochim. Biophys. Acta

    (1983)
  • J.S. Goodwin et al.

    Cell. Immunol

    (1981)
  • W.T. Shearer et al.

    Cell. Immunol

    (1988)
  • J.S. Goodwin et al.

    Clin. Immunol. Immunopathol

    (1980)
  • R.A. Coleman
  • L.L. Brunton et al.

    J. Biol. Chem

    (1976)
  • F. Blotman et al.

    Adv. Prostaglandin Thromboxane Res

    (1980)
  • J.S. Goodwin et al.

    N. Engl. J. Med

    (1977)
  • W.F. Stenson et al.

    J. Immunol

    (1980)
  • Cited by (0)

    Supported in part by the David Fund and a special fund of Texas Children's Hospital.

    View full text